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Titolo:
hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif
Autore:
Ward, DM; Radisky, D; Scullion, MA; Tuttle, MS; Vaughn, M; Kaplan, J;
Indirizzi:
Univ Utah, Dept Pathol, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132 athol, Salt Lake City, UT 84132 USA
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 267, anno: 2001,
pagine: 126 - 134
SICI:
0014-4827(20010701)267:1<126:HIEIMI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMOTYPIC VACUOLE FUSION; PROTEIN COMPLEX; YEAST VACUOLE; AP-3 ADAPTER; SACCHAROMYCES-CEREVISIAE; TRANSPORT; TRAFFICKING; BIOGENESIS; PATHWAY; DOCKING;
Keywords:
Vps41; AP-3; protein sorting; RING-H2 motif;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kaplan, J Univ Utah, Dept Pathol, 50 N Med Dr, Salt Lake City, UT 84132 USA Univ Utah 50 N Med Dr Salt Lake City UT USA 84132 , UT 84132 USA
Citazione:
D.M. Ward et al., "hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif", EXP CELL RE, 267(1), 2001, pp. 126-134

Abstract

Vps41p, the protein encoded by the yeast gene VPS41, has been shown to mediate formation of AP-3 transport vesicles from the Golgi apparatus and to facilitate the docking and fusion of lysosomal vesicles. Although both of these activities involve transient association with membrane structures, the mechanisms that mediate those interactions have not been determined. Orthologues of VPS41 have been identified in humans, Drosophila tomato, and Arabidopsis; the degree of sequence similarity among these genes suggests a highly conserved function. Mere we provide evidence that hVps41, the human homologue of Vps41p, is expressed in two isoforms that differ in that one contains a C-terminal RING-MB sequence motif. Transient expression analysis suggests that this RING-MS domain is responsible for membrane association. Thisobservation was further supported by the cytosolic localization of site-specific mutants. A truncated construct containing only the hVps41 RING-MS domain was found to associate with a class of intracellular vesicles that originated from the Golgi and showed partial coincidence with the delta subunit of the adaptor protein complex-3. Together with information from the homologous yeast system, these results suggest that hVps41 may also be involvedin the formation and fusion of transport vesicles from the Golgi. (C) 2001Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:45:02