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Titolo:
Presence of estrogen receptors in human myeloid monocytic cells (THP-1 cell line)
Autore:
Cutolo, M; Villaggio, B; Bisso, A; Sulli, A; Coviello, D; Dayer, JM;
Indirizzi:
Dept Internal Med, Div Rheumatol, Lab Rheumatol, I-16132 Genoa, Italy DeptInternal Med Genoa Italy I-16132 ab Rheumatol, I-16132 Genoa, Italy ICP, Dept Lab Med, Med Genet Lab, I-20122 Milan, Italy ICP Milan Italy I-20122 ept Lab Med, Med Genet Lab, I-20122 Milan, Italy Univ Hosp Geneva, Dept Internal Med, Div Immunol & Allergy, CH-1211 Geneva, Switzerland Univ Hosp Geneva Geneva Switzerland CH-1211 CH-1211 Geneva, Switzerland
Titolo Testata:
EUROPEAN CYTOKINE NETWORK
fascicolo: 2, volume: 12, anno: 2001,
pagine: 368 - 372
SICI:
1148-5493(200104/06)12:2<368:POERIH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIPHERAL MONOCYTES; RIBONUCLEIC-ACID LEVELS; BINDING-SITES; PHORBOL ESTER; MESSENGER-RNA; T-CELLS; 1,25-DIHYDROXYVITAMIN D-3; INTERSTITIAL COLLAGENASE; INDUCIBLE EXPRESSION; INTERFERON-GAMMA;
Keywords:
THP-1 cells; macrophages; estrogen receptors; estrogens; monocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Cutolo, M Dept Internal Med, Div Rheumatol, Lab Rheumatol, Viale BenedettoXV,6, I-16132 Genoa, Italy Dept Internal Med Viale Benedetto XV,6 Genoa Italy I-16132 taly
Citazione:
M. Cutolo et al., "Presence of estrogen receptors in human myeloid monocytic cells (THP-1 cell line)", EUR CYTOKIN, 12(2), 2001, pp. 368-372

Abstract

Objective: To test THP-1 cells for the presence of estrogen receptors (ER)since studies have demonstrated in viva and irt vitro, the influence of estrogens on cells involved in immune response (i.e. macrophages), and since it has been demonstrated that human myeloid monocytic THP-1. cell acquire phenotypic and functional macrophage-like features after incubation with several cytokines or pharmacological agents. Design: Stimulation of THP-1 cells with phorbol myristate acetate (PMA) to prompt their differentiation intomacrophage-like cells and evaluation of the possible induction of ER, Methods: The expression of ER was: analyzed by immunocytochemical assay, reverse transcriptase polymerase chain reaction(RT-PCR) and Western blot analysis. Results: After stimulation by PMA, the human myeloid monocytic THP-I cells showed the presence of ER, together with markers of monocytic cell differentiation such as CD68, CD54 and HLA-DR, Conclusion: Estrogen effects may be exerted directly through ER on monocytes/macrophages. PMA-treated THP-1 cells may constitute a useful in vitro model to determine the effects of estrogens on macrophage;like cells and their implications in the inflammatory and immune processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:36:36