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Titolo:
The use of microsatellite analysis in Solanum tuberosum L. in vitro plantlets derived from cryopreserved germplasm
Autore:
Harding, K; Benson, EE;
Indirizzi:
DAMAR, Cupar KY15 5RJ, Fife, Scotland DAMAR Cupar Fife Scotland KY15 5RJDAMAR, Cupar KY15 5RJ, Fife, Scotland Univ Abertay Dundee, Sch Sci & Engn, Div Mol & Life Sci, Plant Conservat Biotechnol Grp, Dundee DD1 1HG, Scotland Univ Abertay Dundee Dundee Scotland DD1 1HG rp, Dundee DD1 1HG, Scotland
Titolo Testata:
CRYO-LETTERS
fascicolo: 3, volume: 22, anno: 2001,
pagine: 199 - 208
SICI:
0143-2044(200105/06)22:3<199:TUOMAI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; POTATO SHOOT-TIPS; PLOIDY STABILITY; SOMATIC HYBRIDS; SLOW GROWTH; GENOME; DNA; DIVERSITY; RECOVERY; PRIMERS;
Keywords:
Solanum tuberosum; cryopreservation; microsatellites; genetic stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Harding, K DAMAR, Drum Rd, Cupar KY15 5RJ, Fife, Scotland DAMAR Drum Rd Cupar Fife Scotland KY15 5RJ 5RJ, Fife, Scotland
Citazione:
K. Harding e E.E. Benson, "The use of microsatellite analysis in Solanum tuberosum L. in vitro plantlets derived from cryopreserved germplasm", CRYO-LETT, 22(3), 2001, pp. 199-208

Abstract

This study reports the application of the encapsulation/dehydration cryopreservation and microsatellite analysis to the conservation of Solanum tuberosum cultivars Brodick and Golden Wonder. Cryopreserved shoot-tips were capable of up to 40% shoot and plantlet regeneration in Brodick and > 60 % forGolden Wonder. Microsatellite analysis was used with genomic DNA of GoldenWonder and Desiree to establish DNA sequence length polymorphisms. As the basis of stability assessments this technique was applied to: (i) individual, field-grown, plants of Golden Wonder; (ii) individual Golden Wonder plants derived from a single parental tuber progeny; (iii) plantlets derived from in vitro cultures of Golden Wonder and Brodick and (iv) Golden Wonder and Brodick plantlets derived from cryopreserved germplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:00:53