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Titolo:
IDENTIFICATION OF A BILE-ACID RESPONSE ELEMENT IN THE CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE CYP7A
Autore:
STROUP D; CRESTANI M; CHIANG JYL;
Indirizzi:
NE OHIO UNIV,COLL MED,DEPT BIOCHEM & MOL PATHOL,4209 STATE ROUTE 44,POB 95 ROOTSTOWN OH 44272 NE OHIO UNIV,COLL MED,DEPT BIOCHEM & MOL PATHOL ROOTSTOWN OH 44272
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 2, volume: 36, anno: 1997,
pagine: 508 - 517
SICI:
0193-1857(1997)36:2<508:IOABRE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; EXPRESSION; TAUROCHOLATE; CELLS; CLONING; RATS;
Keywords:
CYTOCHROME P-450; TRANSIENT TRANSFECTION ASSAY; HEP G2 CELLS; TRANSCRIPTIONAL REGULATION; BILE ACID FEEDBACK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
D. Stroup et al., "IDENTIFICATION OF A BILE-ACID RESPONSE ELEMENT IN THE CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE CYP7A", American journal of physiology: Gastrointestinal and liver physiology, 36(2), 1997, pp. 508-517

Abstract

The transcriptional activity of the cholesterol 7 alpha-hydroxylase gene CYP7A is repressed by bile acids. Taurine conjugates of chenodeoxycholate and deoxycholate, but not cholate and ursodeoxycholate, inhibited the CYP7A promoter/luciferase reporter activity in transient transfection assays in Hep G2 cells. A region from nucleotide (nt) -74 to -55 was found to mediate bile acid response. However, deletion of this bile acid response element (BARE-I) enhanced reporter activity but didnot eliminate the bile acid response. This is due to the presence of another BARE-II located in a conserved region between nt -149 and -128. Deletion or mutations of these sequences reduced promoter activity and abolished bile acid repression. This BARE-II shares an identical AGTTCAAG core sequence with BARE-I. Electrophoretic mobility shift assays of BARE-I and BARE-II probes using Hep G2 nuclear extract and the partially purified binding activity of nt -65/-54 DNA-affinity column revealed that the same or a similar nuclear protein might bind to both BAREs. BARE-II is the major BARE involved in the transcriptional repression of the CYP7A gene by hydrophobic bile acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:37:52