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Titolo:
Effects of buprenorphine on immunogenicity and protective efficacy in the guinea pig keratoconjunctivitis model (Sereny test)
Autore:
Hanson, CE; Ruble, GR; Essiet, I; Hartman, AB;
Indirizzi:
Uniformed Serv Univ Hlth Sci, Dept Lab Anim Med, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA Armed Forces Res Inst Med Sci, Bangkok 10400, Thailand Armed Forces Res Inst Med Sci Bangkok Thailand 10400 kok 10400, Thailand Walter Reed Army Med Ctr, Walter Reed Army Inst Res, Dept Enter Infect, Div Communicable Dis & Immunol, Silver Spring, MD 20910 USA Walter Reed Army Med Ctr Silver Spring MD USA 20910 Spring, MD 20910 USA
Titolo Testata:
COMPARATIVE MEDICINE
fascicolo: 3, volume: 51, anno: 2001,
pagine: 224 - 229
SICI:
1532-0820(200106)51:3<224:EOBOIA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPERATION-DESERT-SHIELD; ESCHERICHIA-COLI; IMMUNE-RESPONSES; VACCINE STRAIN; SHIGELLA; ANIMALS; PAIN; VIRG; IDENTIFICATION; CONSTRUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hanson, CE Uniformed Serv Univ Hlth Sci, Dept Lab Anim Med, 4301 Jones Bridge Rd, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci 4301 Jones Bridge Rd Bethesda MD USA 20814
Citazione:
C.E. Hanson et al., "Effects of buprenorphine on immunogenicity and protective efficacy in the guinea pig keratoconjunctivitis model (Sereny test)", COMPAR MED, 51(3), 2001, pp. 224-229

Abstract

Shigellosis is a disease of global proportions, with an estimated 164.7 million episodes annually throughout the world as well as an estimated 1.1 million associated mortalities in developing countries. Due to increasing incidence, and continued emergence of multi-drug resistant strains, Shigella vaccine development is considered a top public health priority. The guinea pig keratoconjunctivitis model, the basis for the Sereny test, remains the most reliable in vivo indicator of virulence of Shigella strains and immunogenicity and protective efficacy of Shigella vaccine candidates. The model is effective in evaluating the ability of Shigella strains to invade the corneal epithelia of guinea pigs and spread to contiguous cells, with the morevirulent strains causing ulcerative keratoconjunctivitis, However, analgesia is not routinely used to relieve this painful condition because of potential immunomodulation and confounding of experimental results. The objective of the study reported here was to evaluate use of buprenorphine hydrochloride as an analgesic during the Sereny test, Local and systemic immune responses were measured in guinea pigs given buprenorphine versus those responses in controls. Results of this study suggest that buprenorphine, administered at an analgesic dose of 0.05 mg/kg of body weight twice daily, can be successfully used with the model without significantly affecting immunologicevaluation of Shigella vaccine candidates. However, in buprenorphine-treated animals, there was a significant increase in the amount of mucopurulent ocular discharge, requiring frequent cleaning of the affected eyes. Additionally, animals treated with buprenorphine had significant reduction in bodyweight, in comparison with saline controls.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:09:29