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Titolo:
Interference of transferrin isoform types with carbohydrate-deficient transferrin quantification in the identification of alcohol abuse
Autore:
Helander, A; Eriksson, G; Stibler, H; Jeppsson, JO;
Indirizzi:
Karolinska Inst, Dept Clin Neurosci, Sect Clin Alcohol & Drug Addict Res, SE-17176 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17176 es, SE-17176 Stockholm, Sweden Karolinska Inst, Dept Clin Neurosci, Sect Neurol, SE-17176 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17176 ol, SE-17176 Stockholm, Sweden Karolinska Hosp, Alcohol Lab, Dept Clin Chem, SE-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden SE-17176 em, SE-17176 Stockholm, Sweden Malmo Univ Hosp, Dept Clin Chem, SE-20502 Malmo, Sweden Malmo Univ Hosp Malmo Sweden SE-20502 Clin Chem, SE-20502 Malmo, Sweden
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 7, volume: 47, anno: 2001,
pagine: 1225 - 1233
SICI:
0009-9147(200107)47:7<1225:IOTITW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; GAMMA-GLUTAMYL-TRANSFERASE; PRIMARY BILIARY-CIRRHOSIS; SYNDROME TYPE-I; DIFFERENTIAL-DIAGNOSIS; GLYCOPROTEIN SYNDROMES; SERUM TRANSFERRIN; LIVER-DISEASE; CONSUMPTION; MARKER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Helander, A Karolinska Hosp, Alcohol Lab, Dept Clin Chem, L7-03, SE-17176 Stockholm, Sweden Karolinska Hosp L7-03 Stockholm Sweden SE-17176 kholm, Sweden
Citazione:
A. Helander et al., "Interference of transferrin isoform types with carbohydrate-deficient transferrin quantification in the identification of alcohol abuse", CLIN CHEM, 47(7), 2001, pp. 1225-1233

Abstract

Background: Isoforms of transferrin interfere with measurement of carbohydrate-deficient transferrin (CDT) as a marker of heavy alcohol consumption. We evaluated the rate of inaccurate CDT results by immunoassays. Methods: We studied 2360 consecutive sera (1614 individuals) submitted forCDT assay without clinical information as well as samples from 1 patient with a congenital disorder of glycosylation (CDG Ia) and from 6 healthy carriers of CDG Ia. The CDTect, %CDT-TIA, and new %CDT immunoassays were compared with HPLC (%CDT-HPLC), Transferrin isoform pattern were evaluated by isoelectric focusing (IEF). Results: Transferrin BC and CD heterozygotes were found at frequencies of similar to0.7% and similar to0.2%, respectively. Another transferrin C subtype, where di- and trisialotransferrin partly coeluted (tentatively identified as C2C3), was observed in similar to0.6%. Compared with the %CDT-HPLC method, the immunoassays often produced low results for transferrin BC and high results for transferrin CD and "C2C3". A very high trisialotransferrin value (frequency similar to1%) often produced high CDT immunoassay results. In four of six healthy carriers of CDG Ia, a- and disialotransferrin were highly increased and the HPLC and IEF isoform patterns were indistinguishable from those in alcohol abuse. Conclusions: Rare transferrin isoform types and abnormal amounts of trisialotransferrin (total frequency similar to2-3%) may cause incorrect determination of CDT with immunoassays, The observed variants were readily identified by HPLC and IEF, which can be recommended for verification of CDT immunoassay results in doubtful cases. In healthy carriers of CDC Ia, CDT is highby all assays. (C) 2001 American Association for Clinical Chemistry.

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Documento generato il 02/12/20 alle ore 17:48:39