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Titolo:
Characterization of recombinant yeast exo-beta-1,3-glucanase (Exg 1p) expressed in Escherichia coli cells
Autore:
Suzuki, K; Yabe, T; Maruyama, Y; Abe, K; Nakajima, T;
Indirizzi:
Tohoku Univ, Grad Sch Agr Sci, Div Appl Life Sci, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 ci, Sendai, Miyagi 9818555, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 6, volume: 65, anno: 2001,
pagine: 1310 - 1314
SICI:
0916-8451(200106)65:6<1310:CORYE(>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; NEUROSPORA-CRASSA; CANDIDA-ALBICANS; PURIFICATION; GENE; WALL; (1->3)-BETA-GLUCANASES; EXO-1,3-BETA-GLUCANASE; SPORULATION; MUTANT;
Keywords:
exo-beta-1,3-glucanase gene (EXG 1); Saccharomyces cerevisiae; recombinant exo-beta-1,3-glucanase (Exg1p);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Nakajima, T Tohoku Univ, Grad Sch Agr Sci, Div Appl Life Sci, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Miyagi 9818555, Japan
Citazione:
K. Suzuki et al., "Characterization of recombinant yeast exo-beta-1,3-glucanase (Exg 1p) expressed in Escherichia coli cells", BIOS BIOT B, 65(6), 2001, pp. 1310-1314

Abstract

Yeast exo-beta -1,3-glucanase gene (EXG1) was expressed in Escherichia coli and the recombinant enzyme (Exg1p) was characterized. The recombinant Exg1p had an apparent molecular mass of 45 kDa by SDS-PAGE and the enzyme has a broad specificity for beta -1,3-linkages as well as beta -1,6-linkages, and also for other beta -glucosidic linked substrates, such as cellobiose and pNPG, Kinetic analyses indicate that the enzyme prefers small substrates such as laminaribiose, gentiobiose, and pNPG rather than polysaccharide substrates, such as laminaran or pustulan, With a high concentration of laminaribiose, the enzyme catalyzed transglucosidation forming laminarioligosaccharides. The enzyme was strongly inhibited with high concentrations of laminaran.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/18 alle ore 09:12:21