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Titolo:
Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display
Autore:
Kimura, T; Nakano, T; Taki, N; Ishikawa, M; Asami, T; Yoshida, S;
Indirizzi:
RIKEN, Inst Phys & Chem Res, Plant Funct Lab, Wako, Saitama 3510198, JapanRIKEN Wako Saitama Japan 3510198 Funct Lab, Wako, Saitama 3510198, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 6, volume: 65, anno: 2001,
pagine: 1275 - 1283
SICI:
0916-8451(200106)65:6<1275:CGEICG>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-EXCHANGE FACTOR; MESSENGER-RNA LEVELS; DELTA-SUBUNIT; SIGNAL-TRANSDUCTION; KINASE HOMOLOG; ALPHA-SUBUNIT; ABSCISIC-ACID; FACTOR EIF-2B; BETA-SUBUNIT; ARABIDOPSIS;
Keywords:
cytokinin; tobacco; differential display; GDP/GTP exchange factor; proline dehydrogenase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, T RIKEN, Inst Phys & Chem Res, Plant Funct Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan RIKEN 2-1 Hirosawa Wako Saitama Japan 3510198 ama 3510198, Japan
Citazione:
T. Kimura et al., "Cytokinin-induced gene expression in cultured green cells of Nicotiana tabacum identified by fluorescent differential display", BIOS BIOT B, 65(6), 2001, pp. 1275-1283

Abstract

The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis andphotosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones, The amino acid sequence encoded by cig2 is similar to the GDP/GTP exchange factor eIF2B, which regulates translation initiation. The expression ofthese cig suggests a complex induction system involving cytokinin and other phytohormones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:33:24