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Titolo:
Marked activation of the N-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations
Autore:
Ueda, N; Liu, Q; Yamanaka, K;
Indirizzi:
Univ Tokushima, Sch Med, Dept Biochem, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 Biochem, Tokushima 7708503, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1-2, volume: 1532, anno: 2001,
pagine: 121 - 127
SICI:
1388-1981(20010531)1532:1-2<121:MAOTNP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOG BRAIN PREPARATIONS; ACYLETHANOLAMINE PHOSPHOLIPIDS; CANNABINOID-RECEPTOR; NEUROBLASTOMA-CELLS; RAT-BRAIN; ANANDAMIDE; BIOSYNTHESIS; ACID; ARACHIDONOYLETHANOLAMINE; ETHANOLAMINE;
Keywords:
phosphodiesterase; phospholipase D; N-acylphosphatidylethanolamine; N-acylethanolamine; anandamide; heart;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ueda, N Kagawa Med Univ, Dept Biochem, 1750-1 Ikenobe, Miki, Kagawa 7610793, Japan Kagawa Med Univ 1750-1 Ikenobe Miki Kagawa Japan 7610793 93, Japan
Citazione:
N. Ueda et al., "Marked activation of the N-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations", BBA-MOL C B, 1532(1-2), 2001, pp. 121-127

Abstract

N-Acylethanolamines including anandamide (an endogenous ligand for cannabinoid receptors) are released from N-acylphosphatidylethanolamine (N-acyl-PE) by the catalysis of a phosphodiesterase of the phospholipase D type. The enzyme was solubilized from the particulate fractions of rat heart with theaid of octyl glucoside, and partially purified by anion-exchange chromatography. The enzyme hydrolyzed N-palmitoyl-PE with a specific activity of 17 nmol/min/mg protein at 37 degreesC. The enzyme activity increased dramatically up to 30-fold by millimolar order of Ca2+. Ca2+ could be replaced with other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+. The hydrolysis of N-arachidonoyl-PE (a precursor of anandamide) was also markedly stimulated by Ca2+. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:14:24