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Titolo:
Is a role of phospholipase A(2) in cholesterol gallstone formation phospholipid species-dependent?
Autore:
Sunami, Y; Tazuma, S; Chayama, K;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Internal Med 1, Minato Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nato Ku, Hiroshima 7348551, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1-2, volume: 1532, anno: 2001,
pagine: 51 - 59
SICI:
1388-1981(20010531)1532:1-2<51:IAROPA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GALLBLADDER BILE; SUPERSATURATED MODEL BILE; FREE FATTY-ACIDS; CRYSTAL-FORMATION; NUCLEATION TIME; BILIARY PHOSPHOLIPASES; NATIVE BILE; SALT; CRYSTALLIZATION; LECITHIN;
Keywords:
cholesterol crystal; phospholipase A(2); phospholipid hydrophobicity; bile lithogenicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Tazuma, S Hiroshima Univ, Sch Med, Dept Internal Med 1, Minato Ku, 1-2-3 Kasumi, Hiroshima 7348551, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348551 8551, Japan
Citazione:
Y. Sunami et al., "Is a role of phospholipase A(2) in cholesterol gallstone formation phospholipid species-dependent?", BBA-MOL C B, 1532(1-2), 2001, pp. 51-59

Abstract

Phospholipase A(2) plays a role in cholesterol gallstone formation by hydrolyzing bile phospholipids into lysolecithin and free fatty acids. This study investigated its effects on cholesterol crystallization in model bile systems. Supersaturated model bile solutions with different cholesterol saturation indexes (1.2, 1.4, and 1.6) were prepared using cholesterol, taurocholate, and egg yolk phosphatidylcholine, soybean phosphatidylcholine, palmitoyl-oleoyl phosphatidylcholine, or palmitoyl-linoleoyl phosphatidylcholine. Then the effect of digestion of phosphatidylcholine by phospholipase A(2) on bile metastability was assessed by spectrophotometry and video-enhanced differential contrast microscopy. Addition of phospholipase A(2) caused therelease of free fatty acids in a time-dependent manner. Cholesterol crystallization was enhanced by an increased crystal growth rate in model bile containing hydrophilic species such as soybean or palmitoyl-linoleoyl phosphatidylcholine, consisting predominantly of polyunsaturated fatty acids. Because phospholipase A(2) enhanced cholesterol crystallization in bile containing hydrophilic phosphatidylcholine species, but not hydrophobic phosphatidylcholine species, release of polyunsaturated fatty acids by hydrolysis maybe responsible for such enhancement. Therefore, the role of phospholipase A(2) in cholesterol gallstone formation depends on the phospholipid speciespresent in bile, so that phospholipid species selection during hepatic excretion is, in part, crucial to the cholesterol stone formation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 22:34:41