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Titolo:
Calorimetrically obtained information about the efficiency of ectoine synthesis from glucose in Halomonas elongata
Autore:
Maskow, T; Babel, W;
Indirizzi:
UFZ Ctr Environm Res Leipzig Halle GmbH, Dept Environm Microbiol, D-04318 Leipzig, Germany UFZ Ctr Environm Res Leipzig Halle GmbH Leipzig Germany D-04318 Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 1-2, volume: 1527, anno: 2001,
pagine: 4 - 10
SICI:
0304-4165(20010702)1527:1-2<4:COIATE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPATIBLE SOLUTES; HALOPHILIC EUBACTERIA; BACTERIA; GROWTH; PROTEINS;
Keywords:
calorimetry; compatible solute; ectoine; yield coefficient; Halomonas elongata;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Maskow, T UFZ Ctr Environm Res Leipzig Halle GmbH, Dept Environm Microbiol, Permoserstr 15, D-04318 Leipzig, Germany UFZ Ctr Environm Res Leipzig Halle GmbH Permoserstr 15 Leipzig Germany D-04318
Citazione:
T. Maskow e W. Babel, "Calorimetrically obtained information about the efficiency of ectoine synthesis from glucose in Halomonas elongata", BBA-GEN SUB, 1527(1-2), 2001, pp. 4-10

Abstract

Compatible solutes are becoming more and more attractive commercially. Thus. knowledge of the efficiency of synthesis of compatible solutes from different carbon substrates is very important. As the growth rate and rates of formation of compatible solutes correspond to the heat flux. calorimetric measurements are particularly suitable for providing this information. By growing microorganisms continuously in a calorimeter. and generating a feeding stream with gradually increasing salinity without changing any other growth conditions, we were able to determine the efficiency of growth-associated synthesis of compatible solutes. This was shown for Halomonas elongata DMSZ 2581(T) growing on glucose, which synthesizes (at 25 degreesC) 1.4,5.6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) as its main osmotic counterweight. The requirement of biologically usable energy for its growth-associated synthesis was found to be very low: a 100% efficiency of the conversion of the substrate-carbon into ectoine is both theoretically possible and was reached approximately in practice. The growth rate and yield coefficient were essentially independent of the ectoine formation rate, and the rate of substrate-carbon assimilation was fat greater than the rate of dissimilation. The specific maximum growth rate was limited by the rate of formation of ectoine. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:31:40