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Titolo:
Investigation of the potential of Maize streak virus to act as an infectious gene vector in maize plants
Autore:
Palmer, KE; Rybicki, EP;
Indirizzi:
Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, W Cape, South Africa Univ Cape Town Rondebosch W Cape South Africa ZA-7701 Cape, South Africa
Titolo Testata:
ARCHIVES OF VIROLOGY
fascicolo: 6, volume: 146, anno: 2001,
pagine: 1089 - 1104
SICI:
0304-8608(2001)146:6<1089:IOTPOM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOVEMENT PROTEIN; COAT PROTEIN; REPLICATIONAL RELEASE; MUTATIONAL ANALYSIS; MOLECULAR-BIOLOGY; STRANDED-DNA; VIRAL-DNA; EXPRESSION; AGROBACTERIUM; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Rybicki, EP Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, W Cape, South Africa Univ Cape Town Rondebosch W Cape South Africa ZA-7701 Africa
Citazione:
K.E. Palmer e E.P. Rybicki, "Investigation of the potential of Maize streak virus to act as an infectious gene vector in maize plants", ARCH VIROL, 146(6), 2001, pp. 1089-1104

Abstract

There has been a great deal of interest in the possibility that geminiviruses might be used as infectious gene vectors for expression of foreign proteins in planes. However, generic mastreviruses such as Maize streak virus (MSV) have no sequences which are dispensable for systemic infection of plants, and there is a strict limitation on the size of viral DNA which can be moved systemically. We attempted to complement the movement functions deleted from a; wild-type-sized, replication-proficient gene replacement vector,by co-infecting plants with it and either wild type MSV, or a replication-deficient but putatively movement-proficient viral construct. While ssDNA formation by the gene replacement vector could be complemented in trans by co-transfected wild type virus, true systemic movement of either the vector,or of co-complementing constructs, did not occur. However, recombination between the two complementing viral constructs frequently occurred to generate wild-type virus genomes. The results therefore suggest that formation ofssDNA and size of the viral replicon are not the sole determinants of whether the MSV movement proteins can mobilise viral sequences and move them systemically in plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:05:07