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Titolo:
Surface binding of aflatoxin B-1 by lactic acid bacteria
Autore:
Haskard, CA; El-Nezami, HS; Kankaanpaa, PE; Salminen, S; Ahokas, JT;
Indirizzi:
RMIT Univ, Sch Med Sci, Key Ctr Appl & Nutr Toxicol, Bundoora, Vic 3083, Australia RMIT Univ Bundoora Vic Australia 3083 icol, Bundoora, Vic 3083, Australia Univ Turku, Dept Biochem & Food Chem, TR-20014 Turku, Finland Univ Turku Turku Finland TR-20014 m & Food Chem, TR-20014 Turku, Finland
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 7, volume: 67, anno: 2001,
pagine: 3086 - 3091
SICI:
0099-2240(200107)67:7<3086:SBOABB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETEROCYCLIC AMINES; REMOVE AFLATOXIN; DAIRY STRAINS; CELL-WALL; LACTOBACILLUS; ABILITY; FOOD; ANTIMUTAGENICITY; MICROORGANISMS; CARCINOGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Haskard, CA Australian Water Qual Ctr, Private Mail Bag 3, Salisbury, SA 5108, Australia Australian Water Qual Ctr Private Mail Bag 3 Salisbury SA Australia 5108
Citazione:
C.A. Haskard et al., "Surface binding of aflatoxin B-1 by lactic acid bacteria", APPL ENVIR, 67(7), 2001, pp. 3086-3091

Abstract

Specific lactic acid bacterial strains remove toxins from liquid media by physical binding. The stability of the aflatoxin B-1 complexes formed with 12 bacterial strains in both viable and nonviable (heat- or acid-treated) forms was assessed by repetitive aqueous extraction. By the fifth extraction, up to 71% of the total aflatoxin B-1 remained bound. Nonviable bacteria retained the highest amount of aflatoxin B-1. Lactobacillus rhamnosus strainGG (ATCC 53103) and L. rhamnosus strain LC-705 (DSM 7061) removed aflatoxin B-1 from solution most efficiently and were selected for further study. The accessibility of bound affatoxin B-1 to an antibody in an indirect competitive inhibition enzyme-linked immunosorbent assay suggests that surface components of these bacteria are involved in binding. Further evidence is the recovery of around 90% of the bound affatoxin from the bacteria by solvent extraction. Autoclaving and sonication did not release any detectable aflatoxin B-1. Variation in temperature (4 to 37 degreesC) and pH (2 to 10) did not have any significant effect on the amount of affatoxin B-1 released, Binding of aflatoxin B-1 appears to be predominantly extracellular for viable and heat-treated bacteria. Acid treatment may permit intracellular binding. In all cases, binding is of a reversible nature, but the stability of the complexes formed depends on strain, treatment, and environmental conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:32:40