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Titolo:
Detection and identification of mammalian reoviruses in surface water by combined cell culture and reverse transcription-PCR
Autore:
Spinner, ML; Di Giovanni, GD;
Indirizzi:
Amer Water Works Serv Co Inc, Qual Control & Res Lab, Belleville, IL 62220USA Amer Water Works Serv Co Inc Belleville IL USA 62220 leville, IL 62220USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 7, volume: 67, anno: 2001,
pagine: 3016 - 3020
SICI:
0099-2240(200107)67:7<3016:DAIOMR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRAHEPATIC BILIARY ATRESIA; POLYMERASE CHAIN-REACTION; NORWALK VIRUS; ENTEROVIRUSES; ADENOVIRUSES; GASTROENTERITIS; GROUNDWATER; POLLUTION; OUTBREAK; RIVERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Spinner, ML Amer Water Works Serv Co Inc, Qual Control & Res Lab, 1115 S Illinois St, Belleville, IL 62220 USA Amer Water Works Serv Co Inc 1115 S Illinois St Belleville IL USA 62220
Citazione:
M.L. Spinner e G.D. Di Giovanni, "Detection and identification of mammalian reoviruses in surface water by combined cell culture and reverse transcription-PCR", APPL ENVIR, 67(7), 2001, pp. 3016-3020

Abstract

Reoviruses are a common class of enteric viruses capable of infecting a broad range of mammalian species, typically with low pathogenicity. Previous studies have shown that reoviruses are common in raw water sources and are often found along with other animal viruses. This suggests that in additionto the commonly monitored enteroviruses, reoviruses might serve as an informative target for monitoring fecal contamination of drinking water sources. Mammalian reoviruses were detected and identified by a combined cell culture-reverse transcription-PCR (RT-PCR) assay with novel primers targeting the W gene that encodes the W major core protein. Five of 26 (19.2%) cytopathic effect-positive cell culture lysates inoculated with surface water werepositive for reoviruses by RT-PCR, DNA sequence analysis of RT-PCR products revealed significant sequence diversity among isolates, which is consistent with the sequence diversity among previously characterized mammalian reoviruses, Sequence analysis revealed persistence of a reovirus genotype at asingle sampling site, while a sample from another site contained two different reovirus genotypes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:10:02