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Titolo:
A NONRADIOACTIVE DNA-PROBE FOR DETECTING DICYANDIAMIDE-DEGRADING SOILBACTERIA
Autore:
TEAUMROONG N; SCHWARZER C; AUER B; HASELWANDTER K;
Indirizzi:
INNSBRUCK UNIV,DEPT MICROBIOL,TECH STR 25 A-6020 INNSBRUCK AUSTRIA INNSBRUCK UNIV,DEPT MICROBIOL A-6020 INNSBRUCK AUSTRIA SURANAREE UNIV TECHNOL,INST AGR TECHNOL,SCH BIOTECHNOL NAKHON RATCHASIMA 30000 THAILAND INNSBRUCK UNIV,DEPT BIOCHEM A-6020 INNSBRUCK AUSTRIA
Titolo Testata:
Biology and fertility of soils
fascicolo: 2, volume: 25, anno: 1997,
pagine: 159 - 162
SICI:
0178-2762(1997)25:2<159:ANDFDD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEIC-ACID HYBRIDIZATION; RAPID METHOD; DEGRADATION; EXTRACTION; SEQUENCES; SEDIMENTS; RECOVERY;
Keywords:
NITRIFICATION INHIBITOR; DICYANDIAMIDE; MYCOBACTERIUM SP; NONRADIOACTIVE DNA PROBE; DNA HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
N. Teaumroong et al., "A NONRADIOACTIVE DNA-PROBE FOR DETECTING DICYANDIAMIDE-DEGRADING SOILBACTERIA", Biology and fertility of soils, 25(2), 1997, pp. 159-162

Abstract

Some soil bacteria are capable of degrading the nitrification inhibitor dicyandiamide (DCD). One of the most efficient isolates is strain EK1 of Mycobacterium sp. for detecting this and closely related DCD-degrading bacteria in soil we developed a non-radioactive DNA probe. A 1.7-kb EK1 DNA fragment was selected from a genomic library and labelledwith digoxigenin. The probe was highly specific for EK1 and closely related or identical species of soil bacteria. A method for direct detection of DNA from soil was developed. The sensitivity of this methodology allowed detection of 2x10(3) EK1 cells g(-1) soil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/06/20 alle ore 13:57:32