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Titolo:
Results of hyperamplification of centrosomes in naturally developing tumors of dogs
Autore:
Setoguchi, A; Okuda, M; Nishida, E; Yazawa, M; Ishizaka, T; Hong, SH; Hisasue, F; Nishimura, R; Sasaki, N; Yoshikawa, Y; Masuda, K; Ohno, K; Tsujimoto, H;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 rnal Med, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomed Sci, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 omed Sci, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Surg, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Vet Surg, Bunkyo Ku, Tokyo 1138657, Japan Univ Cincinnati, Coll Med, Dept Cell Biol Neurobiol & Anat, Cincinnati, OH45267 USA Univ Cincinnati Cincinnati OH USA 45267 l & Anat, Cincinnati, OH45267 USA
Titolo Testata:
AMERICAN JOURNAL OF VETERINARY RESEARCH
fascicolo: 7, volume: 62, anno: 2001,
pagine: 1134 - 1141
SICI:
0002-9645(200107)62:7<1134:ROHOCI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; CHROMOSOME INSTABILITY; GENOMIC INSTABILITY; CELL-CYCLE; GENETIC INSTABILITY; P53-DEFICIENT MICE; MAMMARY-TUMOR; GAMMA-TUBULIN; CANINE; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Tsujimoto, H Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 yo 1138657, Japan
Citazione:
A. Setoguchi et al., "Results of hyperamplification of centrosomes in naturally developing tumors of dogs", AM J VET RE, 62(7), 2001, pp. 1134-1141

Abstract

Objective-To evaluate results of centrosome hyperamplification in naturally developing tumors of dogs. Sample Population-Tumor specimens from 9 dogs with tumors (rhabdomyosarcoma, osteosarcoma, chondrosarcoma, myxosarcoma, and mammary gland tumor) and 2 canine osteosarcoma cell lines. Procedure-3 antibodies for centrosome proteins (ie, anti-gamma -tubulin, anti-BRCA1, and anti-pericentrin) were used for immunohistochemical analysis. Double immunostaining for centrosomes was used to confirm the specificityof these antibodies for centrosomes. Mutational analysis of the canine p53gene was carried out by polymerase chain reaction-single-strand conformation polymorphism analysis, and expression of canine MDM2 protein was evaluated by use of immunohistochemical analysis, using anti-MDM2 antibody. Results-Immunohistochemical analysis of dog osteosarcoma cell lines with apparent aneuploidy revealed frequent hyperamplification of centrosomes in the osteosarcoma cell lines. Similar hyperamplified centrosomes were detected in the tumor tissues from all of the 9 tumors. The frequency of cells with hyperamplified centrosomes (3 to 20/cell) in each tumor tissue ranged from 9.50 to 48.1%, whereas centrosome hyperamplification was not observed in normal lymph nodes from these dogs. In 8 of the 9 tumors, mutation of p53 gene or overexpression of MDM2, or both, was detected. Conclusions and Clinical Relevance-Various types of naturally developing tumors in dogs often have hyperamplification of centrosomes associated with chromosome instability. Hyperamplification of centrosomes is a novel tumor marker for use in cytologic and histologic examinations of clinical specimens obtained from dogs.

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Documento generato il 25/11/20 alle ore 17:52:44