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Titolo:
Evaluation of genetic diversity of poplar genotypes by RAPD and AP-PCR analysis
Autore:
Torjek, O; Kiss, E; Kiss, J; Kondrak, M; Gyulai, G; Gercacz, J; Heszky, L;
Indirizzi:
Szent Istvan Univ, Dept Genet & Plant Breeding, H-2103 Godollo, Hungary Szent Istvan Univ Godollo Hungary H-2103 eeding, H-2103 Godollo, Hungary Hungarian Forest Res Inst, Sarvar Res Stn, H-9600 Sarvar, Hungary Hungarian Forest Res Inst Sarvar Hungary H-9600 , H-9600 Sarvar, Hungary
Titolo Testata:
ACTA BIOLOGICA HUNGARICA
fascicolo: 2-3, volume: 52, anno: 2001,
pagine: 345 - 354
SICI:
0236-5383(2001)52:2-3<345:EOGDOP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARBITRARY PRIMERS; POPULUS; MARKERS;
Keywords:
poplar; RAPD; AP-PCR; polymorphism; cluster analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Heszky, L Szent Istvan Univ, Dept Genet & Plant Breeding, Pater K 1, H-2103 Godollo,Hungary Szent Istvan Univ Pater K 1 Godollo Hungary H-2103 ollo,Hungary
Citazione:
O. Torjek et al., "Evaluation of genetic diversity of poplar genotypes by RAPD and AP-PCR analysis", ACT BIOL HU, 52(2-3), 2001, pp. 345-354

Abstract

RAPD (randomly amplified polymorphic DNA) and AP-PCR (arbitrarily primed PCR) were utilized to establish the genetic diversity of 19 Populus genotypes. A set of 40 primers of random sequence was tested, of which 35 exhibitedpolymorphism. Eighteen primers generated 162 easily detectable bands between 250 and 2500 base pairs in size, sufficient to distinguish between the genotypes. Similarity measures, cluster and multidimensional scaling analysis were performed to evaluate the RAPD and AP-PCR data. Our study demonstrated that in most instances similarity in the RAPD and AP-PCR banding pat terns reflected the relationship due to origin. Nineteen primers gave a species or hybrid-specific pattern. One primer generated a specific pattern in P.euramericana. Ten primers produced specific fragments in VIF (P. alba), 4 primers in KOR (P. pyramidialis x P. berolinensis) and 4 primers in UNA andRAS (P. trichocarpa x P. deltoides). The results of this study demonstrated that RAPD or AP-PCR can be used to distinguish between poplar genotypes.

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Documento generato il 22/01/20 alle ore 06:51:00