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Titolo:
The acute effects of mono(2-ethylhexyl)phthalate (MEHP) on testes of prepubertal Wistar rats
Autore:
Dalgaard, M; Nellemann, C; Lam, HR; Sorensen, IK; Ladefoged, O;
Indirizzi:
Danish Vet & Food Adm, Inst Food Safety & Toxicol, DK-2860 Soborg, DenmarkDanish Vet & Food Adm Soborg Denmark DK-2860 ol, DK-2860 Soborg, Denmark
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 1, volume: 122, anno: 2001,
pagine: 69 - 79
SICI:
0378-4274(20010531)122:1<69:TAEOM(>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM-CELL APOPTOSIS; DI(2-ETHYLHEXYL) PHTHALATE; MONO-(2-ETHYLHEXYL) PHTHALATE; SEMINIFEROUS EPITHELIUM; CLUSTERIN GENE; ORAL TOXICITY; ADULT-RATS; INVOLVEMENT; IDENTIFICATION; DEGENERATION;
Keywords:
DEHP metabolism; vimentin; caspase-3; TUNEL-staining; DNA laddering; TRPM-2 gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Dalgaard, M Danish Vet & Food Adm, Inst Food Safety & Toxicol, Morkhoj Bygade 19, DK-2860 Soborg, Denmark Danish Vet & Food Adm Morkhoj Bygade 19 Soborg Denmark DK-2860
Citazione:
M. Dalgaard et al., "The acute effects of mono(2-ethylhexyl)phthalate (MEHP) on testes of prepubertal Wistar rats", TOX LETT, 122(1), 2001, pp. 69-79

Abstract

A single oral dose of 400 mg/kg body weight of mono(2-ethylhexyl)phthalate(MEHP), the testis toxic metabolite of di(2-ethylhexyl)phthalate. was given to 28-day-old male Wistar rats and the testis toxic effects were investigated 3, 6. and 12 h after exposure. Detachment and sloughing of germ cells were observed, and in the Sertoli cells the cytoplasmatic intermediate filament vimentin collapsed. In the immunohistochemical investigation the androgen receptor distribution was unchanged between the control group and treated groups. The expression of the testosterone-repressed-prostatic-message gene in rat testis increased after 3 hi but returned to control levels after6 and 12 h. Caspase-3 activity increased 3 and 12 h after MEHP exposure. This increase could not be correlated to an increase in DNA fragmentation orincrease in apoptotic numbers of germ cells. In conclusion, the effect of MEHP in testis is apparently not involving the androgen receptor. Vimentin localisation in the Sertoli cells, and increased levels of caspase-3 activity appear to be sensitive and early markers of MEHP testis toxicity. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 11:48:42