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Titolo:
Microcystin-induced down-regulation of lymphocyte functions through reduced IL-2 mRNA stability
Autore:
Yea, SS; Kim, HM; Oh, HM; Paik, KH; Yang, KH;
Indirizzi:
Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea Korea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea Korea Res Inst Biosci & Biotechnol, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea Inje Univ, Paik Inje Mem Inst Biomed Sci, Pusan 614735, South Korea Inje Univ Pusan South Korea 614735 Biomed Sci, Pusan 614735, South Korea
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 1, volume: 122, anno: 2001,
pagine: 21 - 31
SICI:
0378-4274(20010531)122:1<21:MDOLFT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLUE-GREEN-ALGAE; PROTEIN PHOSPHATASES; ADENYLATE-CYCLASE; OKADAIC ACID; INHIBITION; NODULARIN; AERUGINOSA; EXPRESSION; TOXINS; CYANOBACTERIA;
Keywords:
microcystin; nodularin; lymphocytes; interleukin-2; mRNA stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, KH Korea Adv Inst Sci & Technol, Dept Biol Sci, 373-1 Kusong Dong, Taejon 305701, South Korea Korea Adv Inst Sci & Technol 373-1 Kusong Dong Taejon South Korea 305701
Citazione:
S.S. Yea et al., "Microcystin-induced down-regulation of lymphocyte functions through reduced IL-2 mRNA stability", TOX LETT, 122(1), 2001, pp. 21-31

Abstract

Here we report that lymphocyte functions were down-regulated by cyanobacterial hepatotoxin microcystin. Treatment of three microcystin (MC) isotypes,MC-LII. MC-YR and nodularin, on B6C3F1 mouse splenocytes produced dose-dependent inhibition of in vitro polyclonal antibody response and lymphoproliferation to LPS. ConA-induced lymphoproliferative response was decreased by MC-YR and nodularin, but no significant effect was observed in the MC-LR treatment. Intraperitoneal administration of nodularin into B6C3F1 mice decreased humoral immune responses to sheep red blood cell (sRBC), and the inhibitory effect became severe when hepatic uptake of nodularin was blocked by rifampicin. Each MC 1 muM suppressed phorbol 12-myristate 13-acetate (PMA) plus ionomycin-induced IL-2 mRNA expression in splenocytes acid thymocytes,but not in EL-4 mouse thymoma cells. To further characterize the mechanismfor the reduced IL-2 mRNA level, IL-2 mRNA stability was measured using RT-PCR. Deprivation of PMA/ionomycin stimuli from activated splenocytes and blockade of new transcription resulted in destabilization of IL-2 mRNA, which was accelerated by MC treatment. These results demonstrated that MC down-regulated lymphocyte functions and the immunosuppression was mediated. at least in part. through decreased IL-2 mRNA stability. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:32:52