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Titolo:
Establishment of heterotropic liver tissue mass with direct link to the host liver following implantation of hepatocytes transfected with vascular endothelial growth factor gene in mice
Autore:
Ajioka, I; Nishio, R; Ikekita, M; Akaike, T; Sasaki, M; Enami, J; Watanabe, Y;
Indirizzi:
Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan Zenyaku Kogyo Co Ltd, Res Lab, Tokyo 178, Japan Zenyaku Kogyo Co Ltd Tokyo Japan 178 o Co Ltd, Res Lab, Tokyo 178, Japan Sci Univ Tokyo, Dept Appl Biol Sci, Chiba 2641, Japan Sci Univ Tokyo Chiba Japan 2641 o, Dept Appl Biol Sci, Chiba 2641, Japan
Titolo Testata:
TISSUE ENGINEERING
fascicolo: 3, volume: 7, anno: 2001,
pagine: 335 - 344
SICI:
1076-3279(200106)7:3<335:EOHLTM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; HEPATIC REGENERATION; TRANSPLANTATION; RATS; FAILURE; IDENTIFICATION; ANGIOPOIETIN-1; REPLACEMENT; SURVIVAL; DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, Y Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, 4259 Nagatsuda, Yokohama,Kanagawa 2268501, Japan Tokyo Inst Technol 4259 Nagatsuda Yokohama Kanagawa Japan 2268501
Citazione:
I. Ajioka et al., "Establishment of heterotropic liver tissue mass with direct link to the host liver following implantation of hepatocytes transfected with vascular endothelial growth factor gene in mice", TISSUE ENG, 7(3), 2001, pp. 335-344

Abstract

One of the major goals of tissue engineering is to establish an integratedorgan in vivo. We have previously shown that transfection of vascular endothelial growth factor (VEGF) gene into hepatocytes promotes tissue formation by engrafted cells. Here we show that tissue growth was significantly enhanced by co-transplantation of hepatocyte growth factor (HGF) and tumor necrosis factor-alpha (TNF alpha) gene transfected hepatocytes with VEGF-gene transfected cells, but tissue islands were scattered nonspecifically in theabdomen of mice. The result brought us forward to the next step to establish an integrated mass and structural formation of liver tissue. We entrapped VEGF gene transfected hepatocytes in a nylon mesh bag and intraperitoneally engrafted close to the liver. Three weeks later, the bag was covered by a thick network of blood vessels, compared to the control. Histological examination showed that the blood vessels penetrated the parenchyma of the engrafted bag and formed a well-developed vessel network in the region. The use of hepatocytes from lacZ transgenic mice and PCR analysis demonstrated survival and albumin production by hepatocytes in the engrafted bag. Our model can potentially be developed into a heterotropic artificial liver with direct access to the host blood circulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:06:37