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Titolo:
Development of a three-dimensional transmigration assay for testing cell-polymer interactions for tissue engineering applications
Autore:
Gosiewska, A; Rezania, A; Dhanaraj, S; Vyakarnam, M; Zhou, J; Burtis, D; Brown, L; Kong, W; Zimmerman, M; Geesin, JC;
Indirizzi:
Johnson & Johnson Wound Healing Technol Resource, Skillman, NJ 08558 USA Johnson & Johnson Wound Healing Technol Resource Skillman NJ USA 08558 SA Johnson & Johnson Corp Biomat Ctr, Somerville, NJ USA Johnson & Johnson Corp Biomat Ctr Somerville NJ USA , Somerville, NJ USA
Titolo Testata:
TISSUE ENGINEERING
fascicolo: 3, volume: 7, anno: 2001,
pagine: 267 - 277
SICI:
1076-3279(200106)7:3<267:DOATTA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; INTEGRINS; ADHESION; COLLAGEN; MIGRATION; KERATINOCYTES; CHONDROCYTES; FIBRONECTIN; STRENGTH; IMPLANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Gosiewska, A Johnson & Johnson Wound Healing Technol Resource, 199 Grandview Rd, Skillman, NJ 08558 USA Johnson & Johnson Wound Healing Technol Resource 199 Grandview Rd Skillman NJ USA 08558
Citazione:
A. Gosiewska et al., "Development of a three-dimensional transmigration assay for testing cell-polymer interactions for tissue engineering applications", TISSUE ENG, 7(3), 2001, pp. 267-277

Abstract

The ability of synthetic or natural scaffolds to support invasion of cellsfrom surrounding tissue is a key parameter for tissue engineering (TE). Inthis study, the migration of fibroblasts, chondrocytes, and osteoblasts into biodegradable polymer scaffolds was evaluated using a novel, three-dimensional (3-D) transmigration assay. This assay is based on a cell-populated contracted collagen lattice with a biodegradable polymer scaffold implantedat the center of the collagen gel. Cell migration into the scaffolds was assessed both quantitatively and qualitatively following various time lengths in culture using image analysis. Chondrocytes, incorporated within the collagen lattice, migrated into polymer scaffolds, when cultured both statically or in a rotating bioreactor. However, the bioreactor cultures resulted in a significantly greater cell invasion as compared to static cultures. There was a cell density-dependent osteoblast migration from collagen latticeinto polymer scaffold, when tested in the transmigration assay. In addition, polymer scaffolds, treated with or without recombinant human platelet-derived growth factor (rh-PDGF-BB) were evaluated for fibroblast migration. The presence of rh-PDGF-BB resulted in significantly greater fibroblast invasion as compared to untreated scaffolds. Our studies suggest that the transmigration model provides a rapid system for testing cell invasion of potential scaffolds for tissue engineering applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:37:39