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Titolo:
Thrombin-induced MCP-1 expression involves activation of the p22phox-containing NADPH oxidase in human vascular smooth muscle cells
Autore:
Brandes, RP; Viedt, C; Nguyen, K; Beer, S; Kreuzer, J; Busse, R; Gorlach, A;
Indirizzi:
Klinikum JW Goethe Univ, Inst Kardiovasc Physiol, D-60590 Frankfurt, Germany Klinikum JW Goethe Univ Frankfurt Germany D-60590 590 Frankfurt, Germany Univ Heidelberg, Heidelberg, Germany Univ Heidelberg Heidelberg GermanyUniv Heidelberg, Heidelberg, Germany
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 6, volume: 85, anno: 2001,
pagine: 1104 - 1110
SICI:
0340-6245(200106)85:6<1104:TMEIAO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE-CHEMOATTRACTANT PROTEIN-1; SUPEROXIDE ANION PRODUCTION; MESSENGER-RNA EXPRESSION; ANGIOTENSIN-II; NAD(P)H OXIDASE; TYROSINE PHOSPHATASES; SIGNAL-TRANSDUCTION; CRITICAL COMPONENT; ENDOTHELIAL-CELLS; REDOX REGULATION;
Keywords:
oxidative stress; thrombin; NADPH oxidase; p22phox; MCP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Brandes, RP Klinikum JW Goethe Univ, Inst Kardiovasc Physiol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany Klinikum JW Goethe Univ Theodor Stern Kai 7 Frankfurt Germany D-60590
Citazione:
R.P. Brandes et al., "Thrombin-induced MCP-1 expression involves activation of the p22phox-containing NADPH oxidase in human vascular smooth muscle cells", THROMB HAEM, 85(6), 2001, pp. 1104-1110

Abstract

Activation of vascular smooth muscle cells (VMSC) by thrombin induces the expression of the chemokine. monocyte chemoattractant protein-1 (MCP-1). Weinvestigated in cultured human and rat VSMC whether reactive oxygen species (ROS) derived from the vascular NADPH oxidase contribute to this effect. Exposure of cultured VSMC To thrombin rapidly increased ROS formation, phosphorylation of p38 MAP kinase as well as the expression of MCP-1. Specific inhibition of the p22phox subunit of the vascular NADPH oxidase using either p22phox neutralizing antibody or p22phox antisense oligonucleotides attenuated thrombin-induced ROS generation. Furthermore, thrombin-induced p38 MAP kinase activation as well as MCP-1 expression were impaired by antioxidants as well as by p22phox antisense oligonucleotides. Inhibition of p38 MAP kinase diminished the thrombin-induced expression of MCP-1. Conclusion: Thrombin, by activating a p22phox-containing NADPH oxidase. elicits ROS generation and activation of p38 MAP kinase in VSMC. The subsequent induction of MCP-I expression highligts the crucial role of the p22phox-containing NADPHoxidase in thrombin-induced signal transduction in VSMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:05:40