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Titolo:
Recruitment of labelled monocytes by experimental venous thrombi
Autore:
McGuinness, CL; Humphries, J; Waltham, M; Burnand, KG; Collins, M; Smith, A;
Indirizzi:
Univ London Kings Coll, GKT Med Sch, Dept Surg, London SE1 7EH, England Univ London Kings Coll London England SE1 7EH g, London SE1 7EH, England
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 6, volume: 85, anno: 2001,
pagine: 1018 - 1024
SICI:
0340-6245(200106)85:6<1018:ROLMBE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-PLASMINOGEN ACTIVATOR; GROWTH-FACTOR VEGF; CHEMOTACTIC PROTEIN-1; MIGRATION; ORGANIZATION; RESOLUTION; ADHESION; CANCER; INVIVO; MCP-1;
Keywords:
venous; thrombosis; monocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, A Univ London Kings Coll, GKT Med Sch, Dept Surg, 1st Floor,North Wing,St Thomas Campus, London SE1 7EH, England Univ London Kings Coll 1st Floor,North Wing,St Thomas Campus London England SE1 7EH
Citazione:
C.L. McGuinness et al., "Recruitment of labelled monocytes by experimental venous thrombi", THROMB HAEM, 85(6), 2001, pp. 1018-1024

Abstract

Objective. Infusing monocytes that have been stimulated to produce fibrinolytic activators and factors that regulate cell proliferation, migration and maturation, might enhance venous thrombus resolution. The aim of this study was to determine the time course of infused monocyte recruitment into venous thrombus in an appropriate model of this di;ease. Design and Methods. Thrombus was induced in the inferior vena cava of male Wistar rats using reduced flow (80-90% stenosis). The vessel wall was examined at 1hr by scanning electron microscopy. Resolving thrombi with surrounding vena cava were obtained at 1, 7, 14 and 21 days after induction (n = 8). Sections, taken at0.5 mm intervals (10-15 sections per thrombus), were stained using haematoxylin, Martius Scarlet Blue and antibodies against monocytes, platelets andfibrin. Sections from human venous thrombi (n = 4) were similarly stained. The area occupied by monocytes tin relative pixel units. RPU) was determined using computer aided image analysis, Peripheral rat blood monocytes wereextracted. fluorescently labelled and injected intravenously into 7 rats prior to thrombus induction, Vena cava with thrombus was harvested 1 h, 2, 3, 4, 7, 14 and 25 days after induction and their fluorescence measured. Thefluorescent content of the caval wall and thrombus was analysed in greaterdetail at 2 and 25 days after thrombus induction (n = 4 at each time interval), Results. Experimental thrombi were structurally similar to human thrombus and resolved within 14-21 days. Scanning electron microscopy showed minimal endothelial damage at I h with signs of early thrombus formation (platelet, red cell leukocyte and fibrin deposition), Neutrophils were the predominant leukocyte in the thrombus at 1 day, with monocytes making up only 0.3% (0.04% sem) of the area of the thrombus. There was a steady increase inthrombus monocyte content and by 21 days the percentage area of thrombus covered by monocytes had increased by over 35 fold to 11.5% (2.3% sam) (p < 0.001). Initially, monocytes appeared around the edge of the thrombus and became more evenly distributed through the thrombus as resolution progressed. Labelled monocytes could be found in the circulation up to I week after infusion. The fluorescent content (RPU) of the thrombus increased over 25 days (mean RPU At 2 days 0.012. sem 0.005: mean RPU at 25 days 1.062. sem 0.252, p = 0.008). The number of labelled monocytes in the vessel wall peaked at 2 days and decreased thereafter. Conclusion. The structure of thrombi produced by this model was comparable to that of human venous thrombi. Endogenous and injected monocytes migrated into the thrombus during natural resolution, possibly via the vein wall. Monocyte targeting could therefore be used to develop novel treatments for venous thrombosis. with the aim of reducing post-thrombotic complications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 17:22:25