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Titolo:
Detection of target DNA by electrochemical method
Autore:
Maruyama, K; Motonaka, J; Mishima, Y; Matsuzaki, Y; Nakabayashi, I; Nakabayashi, Y;
Indirizzi:
Univ Tokushima, Dept Chem Sci & Technol, Fac Engn, Tokushima 7708506, Japan Univ Tokushima Tokushima Japan 7708506 ac Engn, Tokushima 7708506, Japan Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan Univ Tokushima Tokushima Japan 7708505 eut Sci, Tokushima 7708505, Japan Kansai Univ, Fac Engn, Suita, Osaka 5648680, Japan Kansai Univ Suita Osaka Japan 5648680 c Engn, Suita, Osaka 5648680, Japan Kansai Univ, HRC, Suita, Osaka 5648680, Japan Kansai Univ Suita Osaka Japan 5648680 v, HRC, Suita, Osaka 5648680, Japan
Titolo Testata:
SENSORS AND ACTUATORS B-CHEMICAL
fascicolo: 1-3, volume: 76, anno: 2001,
pagine: 215 - 219
SICI:
0925-4005(20010601)76:1-3<215:DOTDBE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOSENSOR; VOLTAMMETRY; ELECTRODE; SEQUENCES;
Keywords:
DNA; osmium complex; electrochemical detection; square wave voltammetry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Engineering, Computing & Technology
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Maruyama, K Univ Tokushima, Dept Chem Sci & Technol, Fac Engn, Minamijosanjima 2-1, Tokushima 7708506, Japan Univ Tokushima Minamijosanjima 2-1 Tokushima Japan 7708506 an
Citazione:
K. Maruyama et al., "Detection of target DNA by electrochemical method", SENS ACTU-B, 76(1-3), 2001, pp. 215-219

Abstract

The interaction of the osmium complexes {[Os(bpy)(3)](3+) (bpy = bipyridine), [Os(phen)(3)](2+) (phen = 1,10-phenanthroline), [Os(5,6-dmphen)(3)](2+)(5,6-dmphen = 5,6-dimethyl-1, 10-phenanthroline), and [Os(4,7-dmphen)(3)](2+) (4,7-dmphen = 4,7-dimethyl- 1,10-phenanthroline)} with calf thymus DNA were studied electrochemically. The binding constant of [Os(5,6-dmphen)(3)](2+), K = 4.2 x 10(5) M-1 was found to be larger than those observed for the known metal complex indicator and osmium complexes with methyl phenanthroline ligands. This paper shows that a DNA sensor system with [Os(5,6-dmphen)(3)](2+) as a hybridization indicator and square wave voltammetry, could selectively detect DNA in a range of 6.9 x 10(-10) to 6.9 x 10(-5) g/ml. (C)2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 23:59:10