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Titolo:
Toxicologic damage of gas phase cigarette smoke on cells and the protective effect of green tea polyphenols
Autore:
Gao, JT; Tang, HR; Zhao, BL;
Indirizzi:
Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China Acad Sinica Beijing Peoples R China 100101 ijing 100101, Peoples R China Food Res Inst, Norwich NR4 7NA, Norfolk, England Food Res Inst Norwich Norfolk England NR4 7NA h NR4 7NA, Norfolk, England
Titolo Testata:
RESEARCH ON CHEMICAL INTERMEDIATES
fascicolo: 3, volume: 27, anno: 2001,
pagine: 269 - 279
SICI:
0922-6168(2001)27:3<269:TDOGPC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INITIATION; CANCER;
Keywords:
cigarette smoke; spin label; spin trap; lipid peroxidation; green tea polyphenols; electron spin resonance; free radicals;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Zhao, BL Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China Acad Sinica Beijing Peoples R China 100101 101, Peoples R China
Citazione:
J.T. Gao et al., "Toxicologic damage of gas phase cigarette smoke on cells and the protective effect of green tea polyphenols", RES CHEM IN, 27(3), 2001, pp. 269-279

Abstract

A mouse fibroblast cell line, Chinese hamster lung V-79 cell, was used to assess the toxicological effect of gas phase cigarette smoke (GPCS) on cells and the protective effect of green tea polyphenols (GTP). Exposure of thecultured V-79 cells to GPCS decreased cell viability and caused lipid peroxidation of cell membrane as measured by thiobarbituric acid reaction. Electron spin resonance (ESR) spin trapping and spin labeling studies indicatedthat GPCS exposure could increase the fluidity in the polar surface of cell membrane, but the membrane fluidity in the hydrophobic region was not affected. The conformation of sulfhydryl binding sites on membrane was changed. GTP itself had no effect on the polar surface of membrane nor changed theconformation of sulfhydryl binding sites on membrane proteins in the concentration used in this experiment. Incubation of V-79 cells with GTP aqueoussolution before GPCS treatment could decrease cell death and lipid peroxidation and inhibit the changes of the biophysical properties of cell membrane induced by GPCS in a dose-dependent manner. Also GTP could scavenge the free radicals generated by GPCS. These results indicate that GPCS can inducelipid peroxidation and affect the biophysical properties of cell membrane,which may account for the toxicity of GPCS, and that GTP can prevent thesechanges possibly by scavenging the free radicals in GPCS and inhibiting the membrane lipid peroxidation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:58:02