Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulation of microcin C51 operon expression: the role of global regulators of transcription
Autore:
Fomenko, D; Veselovskii, A; Khmel, I;
Indirizzi:
Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia Russian Acad Sci Moscow Russia 123182 t Mol Genet, Moscow 123182, Russia
Titolo Testata:
RESEARCH IN MICROBIOLOGY
fascicolo: 5, volume: 152, anno: 2001,
pagine: 469 - 479
SICI:
0923-2508(200106)152:5<469:ROMCOE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAMP RECEPTOR PROTEIN; ESCHERICHIA-COLI; RNA-POLYMERASE; H-NS; SUBUNIT SYNTHESIS; STATIONARY-PHASE; PROMOTER; GENE; SIGMA(S); CLONING;
Keywords:
microcin C51 operon; promoter; stationary phase; global regulators of transcription; sigma S factor; CRP; H-NS; Lrp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Khmel, I Russian Acad Sci, Inst Mol Genet, Kurchatov Sq 2, Moscow 123182, Russia Russian Acad Sci Kurchatov Sq 2 Moscow Russia 123182 182, Russia
Citazione:
D. Fomenko et al., "Regulation of microcin C51 operon expression: the role of global regulators of transcription", RES MICROB, 152(5), 2001, pp. 469-479

Abstract

Expression of the microcin C51 operon in Escherichia coli cells is regulated as a function of the phase of growth; it is stimulated during the decelerating phase of growth. Using single-copy P-mcc-lac transcriptional fusion (the promoter region of the microcin C51 operon fused to a promoterless lacoperon in A phage), we showed that transcription from the microcin operon promoter is dependent on sigma (s) (BpoS) factor However, some level of P-mcc-lac expression is possible in rpoS null mutants, indicating that anothersigma factor might be involved in transcription of the microcin C51 operon. Overproduction of sigma (70) decreased P-mcc-directed transcription, presumably as a result of competition of sigma factors for the limited amount of core RNA polymerase. The cyclic AMP-CRP complex was shown to stimulate transcription from P-mcc: the absence of CRP or cAMP in crp or cya mutant cells strongly decreased the level of P-mcc-lac expression. The production of C51 microcin decreased or was absent in rpoS, crp and cya mutant cells. Leucine-responsive protein Lrp and histone-like protein H-NS repressed P-mcc-lac expression in the exponential and decelerating phases of growth. In studies of P-mcc-lac expression in double mutant cells, we showed that proteinsCRP, Lrp and H-NS acted in rpoS-dependent and rpoS-independent ways in transcription of the microcin C51 operon. Mutation hns(-) resulted in an increase in P-mcc-lac expression in crp, rpoS and lrp mutant cells, as in wild-type cells. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 07:42:05