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Titolo:
Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase
Autore:
Liu, JG; Cong, W; Wang, S; Ouyang, F;
Indirizzi:
Chinese Acad Sci, Inst Chem Met, Natl Lab Biochem Engn, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 100080, Peoples R China Shandong Univ, Dept Biol Sci, Shandong 270001, Peoples R China Shandong Univ Shandong Peoples R China 270001 ng 270001, Peoples R China
Titolo Testata:
REACTIVE & FUNCTIONAL POLYMERS
fascicolo: 1-3, volume: 48, anno: 2001,
pagine: 75 - 84
SICI:
1381-5148(20010502)48:1-3<75:SOPABB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFINITY LIGANDS; CHROMATOGRAPHY; AGAROSE; ENZYMES;
Keywords:
poly(vinyl acetate-co-diviyl benzene); stability; immobilization; penicillin acylase; kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, JG Chinese Acad Sci, Inst Chem Met, Natl Lab Biochem Engn, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 Peoples R China
Citazione:
J.G. Liu et al., "Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase", REACT FUNCT, 48(1-3), 2001, pp. 75-84

Abstract

Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmolg(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equalto 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:52:46