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Titolo:
Increased throughput in quantitative bioanalysis using parallel-column liquid chromatography with mass spectrometric detection
Autore:
Jemal, M; Huang, M; Mao, Y; Whigan, D; Powell, ML;
Indirizzi:
Bristol Myers Squibb Co, Pharmaceut Res Inst, Bioanal Res Metab & Pharmacokinet, New Brunswick, NJ 08903 USA Bristol Myers Squibb Co New Brunswick NJ USA 08903 runswick, NJ 08903 USA
Titolo Testata:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
fascicolo: 12, volume: 15, anno: 2001,
pagine: 994 - 999
SICI:
0951-4198(2001)15:12<994:ITIQBU>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; TURBULENT-FLOW CHROMATOGRAPHY; SAMPLE PREPARATION; DIRECT-INJECTION; HUMAN PLASMA; MILLILITER CONCENTRATIONS; ELECTROSPRAY SOURCE; BIOLOGICAL SAMPLES; HUMAN URINE; PHARMACEUTICALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Jemal, M Bristol Myers Squibb Co, Pharmaceut Res Inst, Bioanal Res Metab &Pharmacokinet, POB 191, New Brunswick, NJ 08903 USA Bristol Myers Squibb Co POB 191 New Brunswick NJ USA 08903 03 USA
Citazione:
M. Jemal et al., "Increased throughput in quantitative bioanalysis using parallel-column liquid chromatography with mass spectrometric detection", RAP C MASS, 15(12), 2001, pp. 994-999

Abstract

The feasibility of quantitative bioanalysis by parallel-column liquid chromatography in conjunction with a conventional single-source electrospray mass spectrometer has been investigated using plasma samples containing a drug and its three metabolites. Within a single chromatographic run time, sample injections were made alternately onto each of two analytical columns in parallel at specified intervals, with a mass spectrometer data file opened at every injection. Thus, the mass spectrometer collected data from two sample injections into separate data files within a single chromatographic runtime. Therefore, without sacrificing the chromatographic separation or theselected reaction monitoring (SRM) dwell time, the sample throughput was increased by a factor of two. Comparing the method validation results obtained using the two-column system with those obtained using the corresponding conventional single-column approach, the methods on the two systems were found to be equivalent in terms of accuracy and precision. The parallel-column system is simple and can be implemented using existing laboratory equipment with no additional capital outlays. A parallel-column system configured in this manner can be used not only for the within-a-run analysis of two samples containing two different sets of chemical entities, but also for the within-a-run analysis of two samples containing the same set of chemical entities. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:56:30