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Titolo:
Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones
Autore:
Turner, NA; Gaskin, DJH; Yagnik, AT; Littlechild, JA; Vulfson, EN;
Indirizzi:
AFRC, Inst Food Res, Norwich NR4 7UA, Norfolk, England AFRC Norwich Norfolk England NR4 7UA s, Norwich NR4 7UA, Norfolk, England Univ Exeter, Sch Chem, Exeter EX4 4QD, Devon, England Univ Exeter Exeter Devon England EX4 4QD , Exeter EX4 4QD, Devon, England Univ Exeter, Sch Biol Sci, Exeter EX4 4QD, Devon, England Univ Exeter Exeter Devon England EX4 4QD , Exeter EX4 4QD, Devon, England
Titolo Testata:
PROTEIN ENGINEERING
fascicolo: 4, volume: 14, anno: 2001,
pagine: 269 - 278
SICI:
0269-2139(200104)14:4<269:EORRML>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVE-SITE MODEL; CANDIDA-RUGOSA LIPASE; ALCOHOL REACTS FASTER; PIG-LIVER ESTERASE; SUBSTRATE-BINDING; ORGANIC-SOLVENTS; CATALYZED TRANSESTERIFICATION; PSEUDOMONAS-CEPACIA; HUMICOLA-LANUGINOSA; STRUCTURAL BASIS;
Keywords:
biotransformation; molecular modelling; protein engineering; recombinant lipase; transesterification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Vulfson, EN AFRC, Inst Food Res, Norwich Res Pk, Norwich NR4 7UA, Norfolk,England AFRC Norwich Res Pk Norwich Norfolk England NR4 7UA k, England
Citazione:
N.A. Turner et al., "Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones", PROTEIN ENG, 14(4), 2001, pp. 269-278

Abstract

Enantioselectivity of enzyme catalysis is often rationalized via active site models. These models are constructed on the basis of comparing the enantiomeric excess of product observed in a series of reactions which are conducted with a range of homologous substrates, typically carrying various sidechain substitutions. Surprisingly the practical application of these simple but informative 'pocket size' models has been rarely tested in genetic engineering experiments. In this paper we report the construction, purification and enantioselectivity of two recombinant Rhizomucor miehei lipases which were designed to check the validity of such a model in reactions of ring opening of oxazolin-5(4H)-ones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:53:03