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Titolo:
Transcription factor RF2a alters expression of the rice tungro bacilliformvirus promoter in transgenic tobacco plants
Autore:
Petruccelli, S; Dai, S; Carcamo, R; Yin, YH; Chen, SY; Beachy, RN;
Indirizzi:
Scripps Clin & Res Inst, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 nst, La Jolla, CA 92037 USA Donald Danforth Plant Sci Ctr, Clayton, MO 63105, Australia Donald Danforth Plant Sci Ctr Clayton MO Australia 63105 63105, Australia Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China Chinese AcadSci Beijing Peoples R China 100101 100101, Peoples R China
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 13, volume: 98, anno: 2001,
pagine: 7635 - 7640
SICI:
0027-8424(20010619)98:13<7635:TFRAEO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
YELLOW MOTTLE VIRUS; CAULIFLOWER MOSAIC-VIRUS; CIS-ACTING ELEMENTS; GENE-EXPRESSION; FUNCTIONAL DISSECTION; SALICYLIC-ACID; DNA-BINDING; ACTIVATION; TISSUE; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Beachy, RN Scripps Clin & Res Inst, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 la, CA 92037 USA
Citazione:
S. Petruccelli et al., "Transcription factor RF2a alters expression of the rice tungro bacilliformvirus promoter in transgenic tobacco plants", P NAS US, 98(13), 2001, pp. 7635-7640

Abstract

The promoter from rice tungro bacilliform badnavirus (RTBV) is expressed only in phloem tissues in transgenic rice plants. RF2a, a b-Zip protein fromrice, is known to bind to the Box II Eis element near the TATA box of the promoter. Here, we report that the full-length RTBV promoter and a truncated fragment E of the promoter, comprising nucleotides -164 to +45. result inphloem-specific expression of beta -glucuronidase (GUS) reporter genes in transgenic tobacco plants. When a fusion gene comprising the cauliflower mosaic virus 35S promoter and RF2a cDNA was coexpressed with the GUS reportergenes. GUS activity was increased by 2-20-fold. The increase in GUS activity was positively correlated with the amount of RF2a, and the expression pattern of the RTBV promoter was altered from phloem-specific to constitutive. Constitutive expression of RF2a did not induce morphological changes in the transgenic plants. In contrast, constitutive overexpression of the b-ZIPdomain of RF2a had a strong effect on the development of transgenic plants. These studies suggest that expression of the b-Zip domain can interfere with the function of homologues of RF2a that regulate development of tobaccoplants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:04:58