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Titolo:
Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation
Autore:
Ivanova, PT; Cerda, BA; Horn, DM; Cohen, JS; McLafferty, FW; Brown, HA;
Indirizzi:
Cornell Univ, Dept Mol Med, Ctr Vet Med, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 l Med, Ctr Vet Med, Ithaca, NY 14853 USA Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 t Chem & Chem Biol, Ithaca, NY 14853 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 13, volume: 98, anno: 2001,
pagine: 7152 - 7157
SICI:
0027-8424(20010619)98:13<7152:EIMSAO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; ULTRAVIOLET DETECTION; QUANTITATIVE-ANALYSIS; MEMBRANE-FUSION; PLASMA-MEMBRANE; LIPIDS;
Keywords:
mast cells; phospholipases; exocytosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, HA Cornell Univ, Dept Mol Med, Ctr Vet Med, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 Vet Med, Ithaca, NY 14853 USA
Citazione:
P.T. Ivanova et al., "Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation", P NAS US, 98(13), 2001, pp. 7152-7157

Abstract

Biological membranes contain an extraordinary diversity of lipids. Phospholipids function as major structural elements of cellular membranes, and analysis of changes in the highly heterogeneous mixtures of lipids found in eukaryotic cells is central to understanding the complex functions in which lipids participate. Phospholipase-catalyzed hydrolysis of phospholipids often follows cell surface receptor activation. Recently, we demonstrated that granule fusion is initiated by addition of exogenous, nonmammalian phospholipases to permeabilized mast cells. To pursue this finding, we use positiveand negative mode Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) to measure changes in the glycerophospholipid composition oftotal lipid extracts of intact and permeabilized RBL-2H3 (mucosal mast cell line) cells. The low energy of the electrospray ionization results in efficient production of molecular ions of phospholipids uncomplicated by further fragmentation, and changes were observed that eluded conventional detection methods. From these analyses we have spectrally resolved more than 130 glycerophospholipids and determined changes initiated by introduction of exogenous phospholipase C, phospholipase D, or phospholipase A(2). These exogenous phospholipases have a preference for phosphatidylcholine with long polyunsaturated alkyl chains as substrates and, when added to permeabilized mast cells, produce multiple species of mono- and polyunsaturated diacylglycerols, phosphatidic acids. and lysophosphatidylcholines. respectively. The patterns of changes of these lipids provide an extraordinarily rich source of data for evaluating the effects of specific lipid species generated during cellular processes, such as exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:29:34