Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Preparation and validation of an antibody against gibberellin glucosides
Autore:
Mitani, N; Yang, YY; Nakajima, M; Kamiya, Y; Yokota, T; Yamaguchi, I;
Indirizzi:
Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 iol Chem, Bunkyo Ku, Tokyo 1138657, Japan Pohang Univ Sci & Technol, Dept Biosci, Nam Gu, Pohang 790784, Kyungbuk, South Korea Pohang Univ Sci & Technol Pohang Kyungbuk South Korea 790784 South Korea RIKEN, Plant Sci Ctr, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 nt Sci Ctr, Wako, Saitama 3510198, Japan Teikyo Univ, Dept Biosci, Utsunomiya, Tochigi 3208551, Japan Teikyo Univ Utsunomiya Tochigi Japan 3208551 miya, Tochigi 3208551, Japan
Titolo Testata:
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES
fascicolo: 5, volume: 77, anno: 2001,
pagine: 83 - 86
SICI:
0386-2208(200105)77:5<83:PAVOAA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIOIMMUNOASSAY; CARYOPSES;
Keywords:
gibberellin glucosides; antibody; ELISA; Pharbitis nil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, I Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 yo 1138657, Japan
Citazione:
N. Mitani et al., "Preparation and validation of an antibody against gibberellin glucosides", P JPN AC B, 77(5), 2001, pp. 83-86

Abstract

The preparation and the characteristics of an antibody specific for the 2-O-beta -D-glucosides of C-19-gibberellins are described. The antibody, anti-GA(26)-GEt-antibody, was prepared by immunizing rabbits with an immunogenic conjugate of 2-O-beta -D-glucosyl gibberellin A(26) (GA(26)-GEt) and bovine serum albumin. The cross-reactivities of gibberellin glucosides with theanti-GA(26)-GEt-antibody were examined by enzyme-linked immunosorbent assay (ELISA). While 2-O-beta -D-glucosylated gibberellins such as GA(8)-GEt, GA(26)-GEt, and GA(29)-GEt showed high cross-reactivity to the antibody, others did not. The logit-log standard plots showed the measuring range was from 30 fmol to 10 pmol for the GA(8)-GEt and GA(26)-GEt. The validity of theantibody was examined by the analysis of GA-GEts in immature seeds of Pharbitis nil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 20:13:49