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Titolo:
Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves
Autore:
Egelkrout, EM; Robertson, D; Hanley-Bowdoin, L;
Indirizzi:
N Carolina State Univ, Dept Biochem, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 Biochem, Raleigh, NC 27695 USA N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 ept Bot, Raleigh, NC 27695 USA
Titolo Testata:
PLANT CELL
fascicolo: 6, volume: 13, anno: 2001,
pagine: 1437 - 1452
SICI:
1040-4651(200106)13:6<1437:PCNATI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GOLDEN MOSAIC-VIRUS; TRANSGENIC TOBACCO PLANTS; MAIZE STREAK VIRUS; LARGE T-ANTIGEN; DNA-REPLICATION; RETINOBLASTOMA PROTEIN; DROSOPHILA DEVELOPMENT; MOLECULAR-CLONING; PCNA GENE; TISSUE-SPECIFICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Hanley-Bowdoin, L N Carolina State Univ, Dept Biochem, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 NC 27695 USA
Citazione:
E.M. Egelkrout et al., "Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves", PL CELL, 13(6), 2001, pp. 1437-1452

Abstract

The geminivirus tomato golden mosaic virus (TGMV) amplifies its DNA genomein differentiated plant cells that lack detectable levels of DNA replication enzymes. Earlier studies showed that TGMV induces the accumulation of proliferating cell nuclear antigen (PCNA), the processivity factor for DNA polymerase 6, in mature cells of Nicotiana benthamiana. We sought to determine if PCNA protein accumulation reflects transcriptional activation of the host gene. RNA gel blot analysis detected an similar to 1200-nucleotide PCNAtranscript in young leaves. The same RNA was found in mature leaves of infected but not healthy plants. Reporter gene analysis showed that a 633-bp promoter fragment of the N. benthamiana PCNA gene supports high levels of expression in cultured cells and in young but not mature leaves of healthy transgenic plants. In contrast, PCNA promoter activity was detected in both young and mature leaves of TGMV-infected plants. Developmental studies established a strong relationship between symptom severity, viral DNA accumulation, PCNA promoter activity, and endogenous PCNA mRNA levels. Mutation of anE2F consensus element in the PCNA promoter had no effect on its activity in young leaves but increased transcription in healthy mature leaves. Unlikethe wild-type PCNA promoter, TGMV infection had no detectable effect on the activity of the mutant E2F promoter. Together, these results demonstrate that geminivirus infection induces the accumulation of a host replication factor by activating transcription of its gene in mature tissues, most likely by overcoming E2F-mediated repression.

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Documento generato il 05/04/20 alle ore 11:28:43