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Titolo:
The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture
Autore:
Swiatek, M; Kuras, R; Sokolenko, A; Higgs, D; Olive, J; Cinque, G; Muller, B; Eichacker, LA; Stern, DB; Bassi, R; Herrmann, RG; Wollman, FA;
Indirizzi:
Inst Biol Physicochim, UPR CNRS 1261, F-75005 Paris, France Inst Biol Physicochim Paris France F-75005 S 1261, F-75005 Paris, France Univ Munich, Inst Bot, D-80638 Munich, Germany Univ Munich Munich Germany D-80638 ch, Inst Bot, D-80638 Munich, Germany Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 son Inst Plant Res, Ithaca, NY 14853 USA Univ Paris 07, Inst Jacques Monod, F-75251 Paris, France Univ Paris 07 Paris France F-75251 Jacques Monod, F-75251 Paris, France Univ Verona, Dipartimento Sci & Tecnol, I-37134 Verona, Italy Univ VeronaVerona Italy I-37134 nto Sci & Tecnol, I-37134 Verona, Italy
Titolo Testata:
PLANT CELL
fascicolo: 6, volume: 13, anno: 2001,
pagine: 1347 - 1367
SICI:
1040-4651(200106)13:6<1347:TCGYEA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE NUCLEOTIDE-SEQUENCE; LIGHT-HARVESTING COMPLEX; TRANSFER-RNA GENES; CHLAMYDOMONAS-REINHARDTII; THYLAKOID MEMBRANES; CHLOROPHYLL FLUORESCENCE; HYDROPHOBIC PROTEIN; CHLORELLA-VULGARIS; PETD GENE; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Wollman, FA Inst Biol Physicochim, UPR CNRS 1261, 13 Rue Pierre & Marie Curie, F-75005Paris, France Inst Biol Physicochim 13 Rue Pierre & Marie CurieParis France F-75005
Citazione:
M. Swiatek et al., "The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture", PL CELL, 13(6), 2001, pp. 1347-1367

Abstract

We have characterized the biochemical nature and the function of PsbZ, theprotein product of a ubiquitous open reading frame, which is known as ycf9in Chlamydomonas and ORF 62 in tobacco, that is present in chloroplast andcyanobacterial genomes. After raising specific antibodies to PsbZ from Chlamydomonas and tobacco, we demonstrated that it is a bona fide photosystem II (PSII) subunit, PsbZ copurifies with PSII cores in Chlamydomonas as wellas in tobacco. Accordingly, PSII mutants from Chlamydomonas and tobacco are deficient in PsbZ, Using psbZ-targeted gene inactivation in tobacco and Chlamydomonas, we show that this protein controls the interaction of PSII cores with the light-harvesting antenna; in particular, PSII-LHCII supercomplexes no longer could be isolated from PsbZ-deficient tobacco plants. The content of the minor chlorophyll binding protein CP26, and to a lesser extentthat of CP29, also was altered substantially under most growth conditions in the tobacco mutant and in Chlamydomonas mutant cells grown under photoautotrophic conditions. These PsbZ-dependent changes in the supramolecular organization of the PSII cores with their peripheral antennas cause two distinct phenotypes in tobacco and are accompanied by considerable modificationsin (1) the pattern of protein phosphorylation within PSII units, (2) the deepoxidation of xanthophylls, and (3) the kinetics and amplitude of nonphotochemical quenching. The role of PsbZ in excitation energy dissipation within PSII is discussed in light of its proximity to CP43, in agreement with the most recent structural data on PSII.

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Documento generato il 03/04/20 alle ore 11:01:59