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Titolo:
The method of administration of cholecystokinin determines the effects evoked in the pancreas
Autore:
Ohlsson, B; Borg, K; Rehfeld, JF; Axelson, J; Sundler, F;
Indirizzi:
Univ Lund, Dept Surg, S-22185 Lund, Sweden Univ Lund Lund Sweden S-22185Univ Lund, Dept Surg, S-22185 Lund, Sweden Univ Lund, Dept Physiol Sci, Sect Neuroendocrine Cell Biol, S-22185 Lund, Sweden Univ Lund Lund Sweden S-22185 oendocrine Cell Biol, S-22185 Lund, Sweden Rigshosp, Dept Clin Biochem, DK-2100 Copenhagen, Denmark Rigshosp Copenhagen Denmark DK-2100 Biochem, DK-2100 Copenhagen, Denmark
Titolo Testata:
PANCREAS
fascicolo: 1, volume: 23, anno: 2001,
pagine: 94 - 101
SICI:
0885-3177(200107)23:1<94:TMOAOC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CCK-A RECEPTOR; RAT PANCREAS; CELL-DEATH; APOPTOSIS; BCL-2; BAX; PHOSPHORYLATION; INHIBITION; PROGNOSIS; ALBUMIN;
Keywords:
cholecystokinin; CCK-A receptor; receptor regulation; pancreas; apoptosis; bax; bcl-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ohlsson, B Malmo Univ Hosp, Dept Surg, S-20502 Malmo, Sweden Malmo Univ Hosp Malmo Sweden S-20502 g, S-20502 Malmo, Sweden
Citazione:
B. Ohlsson et al., "The method of administration of cholecystokinin determines the effects evoked in the pancreas", PANCREAS, 23(1), 2001, pp. 94-101

Abstract

Earlier studies have shown different effects on cell proliferation and weight characteristics by sulfated chole-cystokinin-8 (CCK-8S) in the rat pancreas when the peptide has been administered continuously rather than intermittently. The aim of this study was as follows: (i) to compare the effect of continuous infusion and of intermittent injections of CCK-8S on cell proliferation, weight gain, and induction of apoptosis and (ii) to examine the effect of injections of CCK-8S on CCK-A receptor gene expression in the ratpancreas. Male Sprague-Dawley rats had subcutaneous continuous infusion ofCCK-8S in a dose of 5 mug/kg/h or 1% bovine serum albumin (BSA) (vehicle) by implanted osmotic minipumps. The rats were frilled after 4 days. Other rats were either injected subcutaneously only once or injected twice daily for 3 days with either 6 mug Of CCK dissolved in 0.5 mt BSA or 0.5 mt BSA alone. The rats were killed 1, 3, 6, and 12 hours after the last injection. One hour before death they received 5-bromo-2deoxyuridine (BrdU) intraperitoneally to localize and quanti tate the cell proliferation. Plasma was collected for analysis of CCK. The pancreas was dissected and immunohistochemistry alas performed for analysis of the expression of the apoptosis promotingprotein bar and the apoptosis inhibiting protein bcl- 2, and for BrdU and CCK-A receptor localization. In situ hybridization (IS) was used fur examination and semiquantification of CCK-A receptor mRNA expression. Continuous infusion of CCK-8S led to a sixfold increase in plasma. CCK and a 40% increase in pancreatic weight without any effect on BrdU labeling, Immunohistochemistry revealed decreased tissue expression of bax but unaffected expression of bcl-2. Intermittent injections of CCK-8S led to hyper-CCK-emia with increased incorporation of BrdU, indicating increased cell proliferation butno increase in pancreatic weight. Immunohistochemistry showed increased expression of bar, whereas bcl-2 remained unchanged, Immunofluorescence and ISH for the CCK-A receptor and its gene expression, respectively, showed a lowest intensity at 3 hours after CCK-8S injections. The results indicate that decreased apoptosis could explain the increased pancreatic weight duringcontinuous infusion of CCK-8S. An increased apoptosis could explain the lack of pancreatic weight gain upon intermittent injections of CCK-8S despitethe stimulation of cell proliferation, injections of CCK-8S only transiently decreased the tissue levels of its receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 16:49:26