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Titolo:
Evidence of pituitary adenylate cyclase activating polypeptide (PACAP) in pancreatic islet cells by confocal microscopy
Autore:
Petruzzo, P; Cappai, A; Spiga, S; Picciau, S; Serra, G; Fattore, L; Onali, P; Brotzu, G;
Indirizzi:
Policlin Univ Moserrato, Dept Surg Sci & Transplant, I-09042 Cagliari, Italy Policlin Univ Moserrato Cagliari Italy I-09042 , I-09042 Cagliari, Italy Policlin Univ Moserrato, Dept Anim Biol & Ecol, I-09042 Cagliari, Italy Policlin Univ Moserrato Cagliari Italy I-09042 , I-09042 Cagliari, Italy Policlin Univ Moserrato, Dept Neurosci, I-09042 Cagliari, Italy Policlin Univ Moserrato Cagliari Italy I-09042 , I-09042 Cagliari, Italy
Titolo Testata:
PANCREAS
fascicolo: 1, volume: 23, anno: 2001,
pagine: 68 - 71
SICI:
0885-3177(200107)23:1<68:EOPACA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERNALIZATION; SOMATOSTATIN; NEUROTENSIN; RECEPTORS; INSULIN; DEGRADATION; GLUCAGON; BRAIN;
Keywords:
PACAP; pancreatic islet; confocal microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Petruzzo, P Policlin Univ Moserrato, Dept Surg Sci & Transplant, I-09042 Cagliari, Italy Policlin Univ Moserrato Cagliari Italy I-09042 gliari, Italy
Citazione:
P. Petruzzo et al., "Evidence of pituitary adenylate cyclase activating polypeptide (PACAP) in pancreatic islet cells by confocal microscopy", PANCREAS, 23(1), 2001, pp. 68-71

Abstract

Several studies have shown that pituitary adenylate cyclase activating polypeptide (PACAP) stimulates at very low Concentration insulin release from pancreatic beta cells. In addition, PACAP has been evidenced in pancreatic nervous fibers surrounding the islets, the core of the islet, and the capillaries. The aim of the present study was to demonstrate internalization of PACAP in pancreatic islet cells. Pancreatic islets were obtained from Wistar rat pancreata by modified Lacy's isolation method, The isolated islets were incubated in the presence of Fluo-PACAP 27, a fluorescent ligand specific: fur PACAP receptors. At the end of incubation the islets were fixed in poraformaldehyde and then observed by confocal microscope. Fluo-PACAP 27 wasinternalized into pancreatic islet cells, and this process was time- and temperature-dependent (37 degreesC). The fluorescent molecules converged toward the nucleus where an intense fluorescence was evidenced after 60 minutes, Incubation with phenyl arsine oxide as well as with PACAP 6-38, a receptor antagonist, prevented the internalization process. Further studies are required to explain the internalization process of PACAP 27 into the nucleusof pancreatic islet cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:46:10