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Titolo:
Isolation, long-term culture, and characterization of rat pancreatic fibroblastoid/stellate cells
Autore:
Kruse, ML; Hildebrand, PB; Timke, C; Folsch, UR; Schafer, H; Schmidt, WE;
Indirizzi:
Univ Kiel, Dept Med 1, Lab Mol Gastroenterol & Hepatol, D-24105 Kiel, Germany Univ Kiel Kiel Germany D-24105 oenterol & Hepatol, D-24105 Kiel, Germany Ruhr Univ Bochum, St Josef Hosp, Sch Med, Dept Med, D-4630 Bochum, GermanyRuhr Univ Bochum Bochum Germany D-4630 Dept Med, D-4630 Bochum, Germany
Titolo Testata:
PANCREAS
fascicolo: 1, volume: 23, anno: 2001,
pagine: 49 - 54
SICI:
0885-3177(200107)23:1<49:ILCACO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
STELLATE CELLS; IDENTIFICATION; LIVER; ACINI;
Keywords:
experimental pancreatitis; cell culture techniques; stellate cells; myofibroblasts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Kruse, ML Univ Kiel, Dept Med 1, Lab Mol Gastroenterol & Hepatol, Schittenhelmstr 12, D-24105 Kiel, Germany Univ Kiel Schittenhelmstr 12 Kiel Germany D-24105 Kiel, Germany
Citazione:
M.L. Kruse et al., "Isolation, long-term culture, and characterization of rat pancreatic fibroblastoid/stellate cells", PANCREAS, 23(1), 2001, pp. 49-54

Abstract

Investigation of pancreatic interstitial fibroblasts has proven difficult in situ. We have established a method for the isolation of pancreatic fibroblastoid/stellate cells by outgrowth from pancreatic tissue explanted into culture dishes. This technique gives a high yield of viable cells from small tissue samples. Outgrown fibroblastoid cells were established as a primary cell line and characterized during long-term culture. We investigated thedevelopment of stellate cell markers, i.e. fat storage, expression of desmin, and ct-smooth muscle actin (alpha SMA), over weeks in culture. alpha SMA, investigated by indirect immunofluorescence staining and Western blot analysis, revealed a constant rise in expression during routine culture. After 13 passages, approximately 100% of cells were positive for alpha SMA expression, indicating a myofibroblast type of differentiation in vitro.

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Documento generato il 07/04/20 alle ore 22:57:57