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Titolo:
Expression of keratins in normal, immortalized and malignant oral epithelia in organotypic culture
Autore:
Hansson, A; Bloor, BK; Haig, Y; Morgan, PR; Ekstrand, J; Grafstrom, RC;
Indirizzi:
Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Med, S-17177 Stockholm, Sweden Guys Hosp, Dept Oral Med & Pathol, Guys Kings & St Thomas Dent Inst, London, England Guys Hosp London England s Kings & St Thomas Dent Inst, London, England Karolinska Inst, Dept Basic Oral Sci, S-17177 Stockholm, Sweden KarolinskaInst Stockholm Sweden S-17177 Sci, S-17177 Stockholm, Sweden
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 5, volume: 37, anno: 2001,
pagine: 419 - 430
SICI:
1368-8375(200107)37:5<419:EOKINI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; EPIDERMAL-KERATINOCYTES; CYTOKERATIN EXPRESSION; RETINOIC ACID; HUMAN SKIN; DIFFERENTIATION; CELLS; GENE; MUCOSA; PATTERNS;
Keywords:
human oral keratinocytes; organotypic culture; immortalized; malignant; terminal differentiation; immunohistochemistry; keratins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Grafstrom, RC Karolinska Inst, Inst Environm Med, Box 210, S-17177 Stockholm, Sweden Karolinska Inst Box 210 Stockholm Sweden S-17177 lm, Sweden
Citazione:
A. Hansson et al., "Expression of keratins in normal, immortalized and malignant oral epithelia in organotypic culture", ORAL ONCOL, 37(5), 2001, pp. 419-430

Abstract

Keratins have been extensively studied in tissues and cultured keratinocytes but limited information is available on epithelia reconstructed in vitro. The aim of this study was to examine keratin expression in organotypic epithelia with normal (NOK), immortalized (SVpgC2a) and malignant (SqCC/Y1) human buccal cells. Organotypic epithelia were derived from 10 days of culture at the air-liquid interface of collagen gels containing human oral fibroblasts using a standardized serum-free medium. Sections were stained immunohistochemically with selected mono-specific antibodies to a range of keratins. Organotypic epithelia showed sharp differences in keratin expression and distribution. K4/K13, K1/K10, K6/K16 were variably expressed in NOK and SqCC/Y1 but were not detected in SVpgC2a. K5 was expressed in all organotypic epithelia but K14 was absent in SVpgC2a. K7 and K8 showed variable expression while K18 was expressed uniformly in all epithelia. K19 was expressed consistently in NOK and K20 was distributed heterogeneously in SVpgC2a. Overall, organotypic cultures of normal keratinocytes express many of the samekeratins as buccal mucosa. Further, the loss of keratins in SVpgC2a and their retention in SqCC/Y1 have several features in common with the respective keratin profile of oral epithelial dysplasia and well-differentiated oralsquamous cell carcinoma. Although qualitative and quantitative differencesexist compared to keratin expression in vivo, these cell lines in organotypic culture may serve in studies of the multi-step progression of oral cancer. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:38:58