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Titolo:
The YXXQ motif in gp 130 is crucial for STAT3 phosphorylation at Ser727 through an H7-sensitive kinase pathway
Autore:
Abe, K; Hirai, M; Mizuno, K; Higashi, N; Sekimoto, T; Miki, T; Hirano, T; Nakajima, K;
Indirizzi:
Osaka City Univ, Grad Sch Med, Dept Immunol, Abeno Ku, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 unol, Abeno Ku, Osaka 5458585, Japan Osaka City Univ, Grad Sch Med, Dept Ophthalmol & Visual Sci, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 l & Visual Sci, Osaka 5458585, Japan Osaka City Univ, Grad Sch Med, Dept Mol Oncol, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 Dept Mol Oncol, Osaka 5458585, Japan
Titolo Testata:
ONCOGENE
fascicolo: 27, volume: 20, anno: 2001,
pagine: 3464 - 3474
SICI:
0950-9232(20010614)20:27<3464:TYMIG1>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONALLY ACTIVE STAT1; CYTOKINE RECEPTOR GP130; IL-6 RESPONSE ELEMENT; CRE-LIKE SITE; SERINE PHOSPHORYLATION; TYROSINE PHOSPHORYLATION; PROTEIN-KINASE; SIGNAL-TRANSDUCTION; JUNB PROMOTER; GROWTH ARREST;
Keywords:
STAT3; ser phosphorylation; IL-6; gp 130; YXXQ motif; transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Nakajima, K Osaka City Univ, Grad Sch Med, Dept Immunol, Abeno Ku, 1-4-3 Asahi Machi, Osaka 5458585, Japan Osaka City Univ 1-4-3 Asahi Machi Osaka Japan 5458585 , Japan
Citazione:
K. Abe et al., "The YXXQ motif in gp 130 is crucial for STAT3 phosphorylation at Ser727 through an H7-sensitive kinase pathway", ONCOGENE, 20(27), 2001, pp. 3464-3474

Abstract

The signal transducer and activator of transcription (STAT) 3 is essentialfor mediating signals from the receptors for a variety of cytokines and growth factors, including IL-6 and EGF, and from cytoplasmic tyrosine kinases, Upon stimulation, STAT3 is phosphorylated at Ser727 and Tyr705, However, the role of phosphorylation at Ser727, and the kinase pathways responsible for this phosphorylation in IL-6 signaling remain obscure. Here we show that IL-6 activates at least two distinct STAT3 serine kinase pathways and that an H7-sensitive pathway is dominant over a PD98059-sensitive one in HepG2cells stimulated with a low concentration of IL-6, The analysis, using a series of chimeric receptors containing the extracellular domain of the G-CSF receptor, the truncated form of gp 130, and additional short peptides at the gp 130 carboxy-terminus, shelved that the YXXQ motif of gp 130 was sufficient for the H7-sensitive STAT3 Ser727 phosphorylation, This YXXQ-mediated pathway does not involve Erk, p38, JNK, or PKC delta, and requires a sitein the region from 533 to 711 of STAT3 for phosphorylation in vivo. Moreover, we show that Ser727 is required for full transcriptional activity of STAT3 for two different response elements, Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:07:37