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Titolo:
Down-regulation of cathepsin B expression impairs the invasive and tumorigenic potential of human glioblastoma cells
Autore:
Mohanam, S; Jasti, SL; Kondraganti, SR; Chandrasekar, N; Lakka, SS; Kin, Y; Fuller, GN; Yung, AWK; Kyritsis, AP; Dinh, DH; Olivero, WC; Gujrati, M; Ali-Osman, F; Rao, JS;
Indirizzi:
Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 ci, Div Canc Biol, Peoria, IL 61656 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurooncol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 r, Dept Neurooncol, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Neuropathol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 , Dept Neuropathol, Houston, TX 77030 USA Univ Illinois, Coll Med, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 d, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Pathol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 Med, Dept Pathol, Peoria, IL 61656 USA
Titolo Testata:
ONCOGENE
fascicolo: 28, volume: 20, anno: 2001,
pagine: 3665 - 3673
SICI:
0950-9232(20010621)20:28<3665:DOCBEI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GLIOMAS; CYSTATIN-C; PROTEASE INHIBITORS; TUMOR PROGRESSION; CANCER METASTASIS; PROGNOSTIC FACTOR; BREAST-CANCER; ANGIOGENESIS; LOCALIZATION; LINES;
Keywords:
glioblastoma; invasion; cathepsin B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 Canc Biol, Peoria, IL 61656 USA
Citazione:
S. Mohanam et al., "Down-regulation of cathepsin B expression impairs the invasive and tumorigenic potential of human glioblastoma cells", ONCOGENE, 20(28), 2001, pp. 3665-3673

Abstract

Increases in abundance of cathepsin B transcript and protein correlate with increases in tumor grade and alterations in subcellular localization and activity of cathepsin B, The enzyme is able to degrade the components of the extracellular matrix (ECM) and activate other proteases capable of degrading ECM, To investigate the role played by this protease in the invasion ofbrain tumor cells, we transfected SNB19 human glioblastoma cells with a plasmid containing cathepsin B cDNA in antisense orientation. Control cells were transfected with vector alone. Clones expressing antisense cathepsin B cDNA exhibited significant reductions in cathepsin B mRNA, enzyme activity and protein compared to controls. Matrigel Invasion assay showed that the antisense-transfected cells had markedly diminished invasiveness compared with controls. When tumor spheroids containing antisense transfected SNB19 cells expressing reduced cathepsin B were co-cultured with fetal rat brain aggregates, invasion of fetal rat brain aggregates was significantly reduced. Green Fluorescent Protein (GFP) expressing parental cells and antisense transfectants were generated for detection in mouse brain tissue without any post-chemical treatment. Intracerebral injection of SNB19 stable antisense transfectants resulted in reduced tumor formation in nude mice. These results strongly support a role for cathepsin B in the invasiveness of human glioblastoma cells and suggest cathepsin B antisense may prove useful in cancer therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:54:07