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Titolo:
Anti-mitotic properties of indirubin-3'-monoxime, a CDK/GSK-3 inhibitor: induction of endoreplication following prophase arrest
Autore:
Damiens, E; Baratte, B; Marie, D; Eisenbrand, G; Meijer, L;
Indirizzi:
CNRS, Biol Stn, Cell Cycle Grp, F-29682 Roscoff, France CNRS Roscoff France F-29682 Stn, Cell Cycle Grp, F-29682 Roscoff, France Univ Kaiserslautern, Div Food Chem & Environm Toxicol, Dept Chem, D-67663 Kaiserslautern, Germany Univ Kaiserslautern Kaiserslautern Germany D-67663 iserslautern, Germany CNRS, Phytoplankton Ocean Grp, Biol Stn, F-29682 Roscoff, France CNRS Roscoff France F-29682 Ocean Grp, Biol Stn, F-29682 Roscoff, France
Titolo Testata:
ONCOGENE
fascicolo: 29, volume: 20, anno: 2001,
pagine: 3786 - 3797
SICI:
0950-9232(20010628)20:29<3786:APOIAC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASES; CELL-CYCLE; CHEMICAL INHIBITORS; SODIUM-BUTYRATE; CANCER CELLS; DNA; STAUROSPORINE; APOPTOSIS; MEDICINE; MITOSIS;
Keywords:
cyclin-dependent kinase; glycogen synthase kinase; GSK-3 beta; indirubin; kinase inhibitor; cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Meijer, L CNRS, Biol Stn, Cell Cycle Grp, BP 74, F-29682 Roscoff, France CNRS BP 74 Roscoff France F-29682 P 74, F-29682 Roscoff, France
Citazione:
E. Damiens et al., "Anti-mitotic properties of indirubin-3'-monoxime, a CDK/GSK-3 inhibitor: induction of endoreplication following prophase arrest", ONCOGENE, 20(29), 2001, pp. 3786-3797

Abstract

The bis-indole indirubin is the active ingredient of the Traditional Chinese Medicine recipe Danggui Longhui Wan used against chronic myelocytic leukemia, We have previously shown that indirubins are potent inhibitors of cyclin-dependent kinases and glycogen synthase kinase-3, We here investigated the anti-mitotic properties of this class of compounds using the cell permeable indirubin-3 ' -monoxime and the HBL-100 cell line. Indirubin-3 ' -monoxime reversibly arrests asynchronous HBL-100 cells in G2, This arrest is not accompanied by any significant change in expression of the major cell cycle regulators, However indirubin-3 ' -monoxime inhibits the phosphorylationof consensus CDK phosphorylation sites as well as of nucleolin at a specific CDK1/cyclin B phosphorylation site, suggesting a direct action on the mitotic CDK1/cyclin B, When indirubin-3 ' -monoxime is added to HBL-100 cellssynchronized in M phase by nocodazole, cells undergo an endoreplication leading to an 8n DNA content. As soon as indirubin-3 ' -monoxime is washed away, these polyploid cells become aneuploid and later die from necrosis, This mechanism of endoreplication followed by cell death may contribute to theantitumour properties of indirubins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:47:48