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Titolo:
Decreased expression of the NADH : ubiquinone oxidoreductase (complex I) subunit 4 in 1-methyl-4-phenylpyridinium-treated human neuroblastoma SH-SY5Ycells
Autore:
Conn, KJ; Ullman, MD; Eisenhauer, PB; Fine, RE; Wells, JM;
Indirizzi:
VA Med Ctr, Dept Vet Affairs, Bedford, MA 01730 USA VA Med Ctr Bedford MAUSA 01730 , Dept Vet Affairs, Bedford, MA 01730 USA Univ Massachusetts, Sch Med, Shriver Ctr, Div Biomed Sci, Waltham, MA 02254 USA Univ Massachusetts Waltham MA USA 02254 Biomed Sci, Waltham, MA 02254 USA Boston Univ, Sch Med, Dept Psychiat, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 Med, Dept Psychiat, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Neurol, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 ch Med, Dept Neurol, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 h Med, Dept Biochem, Boston, MA 02118 USA
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 3, volume: 306, anno: 2001,
pagine: 145 - 148
SICI:
0304-3940(20010629)306:3<145:DEOTN:>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALZHEIMERS-DISEASE; GENE-EXPRESSION; PARKINSONISM; MITOCHONDRIAL; DEHYDROGENASE; N-METHYL-4-PHENYLPYRIDINE; NEUROTOXICITY; DEGENERATION; PRODUCT; DEATH;
Keywords:
Parkinson's disease; 1-methyl-4-phenyl-pyridinium; reverse transcription-polymerase chain reaction; complex I; NADH : ubiquinone oxidoreductase subunit 4; N-tert-butyl-alpha-(2-sulfophenyl)-nitrone; MPP+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Conn, KJ VA Med Ctr, Dept Vet Affairs, 200 Springs Rd,Bldg 18, Bedford, MA01730 USA VA Med Ctr 200 Springs Rd,Bldg 18 Bedford MA USA 01730 01730 USA
Citazione:
K.J. Conn et al., "Decreased expression of the NADH : ubiquinone oxidoreductase (complex I) subunit 4 in 1-methyl-4-phenylpyridinium-treated human neuroblastoma SH-SY5Ycells", NEUROSCI L, 306(3), 2001, pp. 145-148

Abstract

Oxidative stress and mitochondrial dysfunction have been implicated in Parkinson's disease (PD) pathology. NADH:ubiquinone oxidoreductase (complex I)(EC 1.6.99.3) enzyme activity is aberrant in both PD and 1-methyl-4-phenylpyridinium (MPP+) models of PD. Reverse transcription polymerase chain reaction of RNA isolated from MPP+-treated human neuroblastoma SH-SY5Y cells identified changes in steady-state mRNA levels of the mitochondrial transcript for subunit 4 of complex I (ND4). Expression of ND4 decreased to nearly 50% after 72 h of MPP+ (1 mM) exposure. The expression of other mitochondrial transcripts did not change significantly under the same conditions. Pre-incubation of cells with the free-radical spin-trap, N-tert-butyl-alpha-(2-sulfophenyl)-nitrone prior to MPP+ exposure, prevented decreases in cell viability and ND4 expression. This suggests that functional defects in complex1 enzyme activity in PD and MPP+ toxicity may result from changes in steady-state mRNA levels and that free radicals may be important in this process. (C) 2001 Published by Elsevier Science Ireland Ltd.

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Documento generato il 20/06/19 alle ore 17:17:03