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Titolo:
Orexin-a activates phospholipase C- and protein kinase C-mediated Ca2+ signaling in dopamine neurons of the ventral tegmental area
Autore:
Uramura, K; Funahashi, H; Muroya, S; Shioda, S; Takigawa, M; Yada, T;
Indirizzi:
Jichi Med Sch, Dept Physiol, Kawachi, Tochigi 3290498, Japan Jichi Med Sch Kawachi Tochigi Japan 3290498 wachi, Tochigi 3290498, Japan Kagoshima Univ, Fac Med, Dept Neuropsychiat, Kagoshima 8908520, Japan Kagoshima Univ Kagoshima Japan 8908520 sychiat, Kagoshima 8908520, Japan Showa Univ, Sch Med, Dept Anat, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 Sch Med, Dept Anat, Tokyo 1428555, Japan Natl Inst Physiol Sci, Lab Intracellular Metab, Okazaki, Aichi 4448585, Japan Natl Inst Physiol Sci Okazaki Aichi Japan 4448585 i, Aichi 4448585, Japan
Titolo Testata:
NEUROREPORT
fascicolo: 9, volume: 12, anno: 2001,
pagine: 1885 - 1889
SICI:
0959-4965(20010703)12:9<1885:OAPCAP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NARCOLEPSY; SLEEP; RATS; STIMULATION; AMPHETAMINE; NUCLEUS; MUTATION; REWARD; CANINE; CELLS;
Keywords:
Ca2+ channel; cytosolic Ca2+ concentration; dopamine neuron; orexin; phospholipase C; protein kinase C; ventral tegmental area; VTA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Yada, T Jichi Med Sch, Dept Physiol, Kawachi, Tochigi 3290498, Japan JichiMed Sch Kawachi Tochigi Japan 3290498 ochigi 3290498, Japan
Citazione:
K. Uramura et al., "Orexin-a activates phospholipase C- and protein kinase C-mediated Ca2+ signaling in dopamine neurons of the ventral tegmental area", NEUROREPORT, 12(9), 2001, pp. 1885-1889

Abstract

The orexin-orexin receptor system has been implicated in the regulation ofwakefulness/sleep states. Behavioral and psychostimulant effects of orexins have also been shown. Mesolimbic dopamine neurons in the ventral tegmental area (VTA) are implicated in the regulation of reward and wakefulness/sleep, In the present study, we examined the effect of orexin-A on cytosolic [Ca2+](i) concentration ([Ca2+]) in the isolated rat VTA dopamine neurons. Orexin-A (10(-12)-10(-8)M) concentration dependently increased [Ca2+](i) in dopamine-containing neurons. The [Ca2+](i) responses to orexin-A were inhibited under Ca2+-free conditions and by blockers of voltage-gated Land N-type [Ca2+](i) channels, nitrendipine and omega -conotoxin, respectively. The [Ca2+](i) responses were also abolished by a phosphatidylcholine-specific phospholipase C inhibitor, D609, and a protein kinase C (PKC) inhibitor, calphostin C. A PKC activator, TPA, mimicked orexin-A in increasing [Ca2+](i). These results indicate that orexin-A increases [Ca2+](i) in VTA dopamine neurons via phosphatidylcholine-specific PLC- and PKC-mediated activation ofL- and N-type Ca2+ channels. This effect may serve as the mechanism by which orexin regulates wakefulness/sleep states and exerts its behavioral and psychostimulant effects. NeuroReport 12:1885-1889 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:37:24