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Titolo:
Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1
Autore:
Hopf, M; Gohring, W; Ries, A; Timpl, R; Hohenester, E;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Blackett Lab, Biophys Sect, London SW7 2BW, England Univ London Imperial Coll Sci Technol & Med LondonEngland SW7 2BW gland Max Planck Inst Biochem, Prot Chem Abt, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 Martinsried, Germany Univ London Imperial Coll Sci Technol & Med, Div Med, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 7, volume: 8, anno: 2001,
pagine: 634 - 640
SICI:
1072-8368(200107)8:7<634:CSAMAO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANE PROTEIN; GREEN FLUORESCENT PROTEIN; GROWTH-FACTOR PRECURSOR; AMINO-ACID SEQUENCE; COLLAGEN TYPE-IV; EPITHELIAL MORPHOGENESIS; LAMININ GAMMA-1; LOCALIZATION; LIPOPROTEIN; HOMOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Hohenester, E Univ London Imperial Coll Sci Technol & Med, Blackett Lab, Biophys Sect, Prince Consort Rd, London SW7 2BW, England Univ London Imperial Coll Sci Technol & Med Prince Consort Rd London England SW7 2BW
Citazione:
M. Hopf et al., "Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1", NAT ST BIOL, 8(7), 2001, pp. 634-640

Abstract

Nidogen, an invariant component of basement membranes, is a multifunctional protein that interacts with most other major basement membrane proteins. Here, we report the crystal structure of the mouse nidogen-l GZ fragment, which contains binding sites for collagen IV and perlecan. The structure is composed of an EGF-like domain and an Ii-stranded beta -barrel with a central helix. The beta -barrel domain has unexpected similarity to green fluorescent protein. A large surface patch on the beta -barrel is strikingly conserved in all metazoan nitrogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:08:52