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Titolo:
Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers
Autore:
Bennink, ML; Leuba, SH; Leno, GH; Zlatanova, J; de Grooth, BG; Greve, J;
Indirizzi:
Univ Twente, Dept Appl Phys, iBME Res Inst, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands NCI, Lab Recptor Biol & Gene Express, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 iol & Gene Express, NIH, Bethesda, MD 20892 USA Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA Univ Mississippi Jackson MS USA 39216 Dept Biochem, Jackson, MS 39216 USA Polytech Univ, Dept Chem Engn Chem & Mat Sci, MetroTech Ctr 6, Brooklyn, NY 11201 USA Polytech Univ Brooklyn NY USA 11201 troTech Ctr 6, Brooklyn, NY 11201 USA
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 7, volume: 8, anno: 2001,
pagine: 606 - 610
SICI:
1072-8368(200107)8:7<606:UINBSS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA; FORCE; MOLECULE; HISTONE; TRANSCRIPTION; POLYMERASE; TEMPLATE; PROTEIN; TENSION; B4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Bennink, ML Univ Twente, Dept Appl Phys, iBME Res Inst, POB 217, NL-7500 AE Enschede, Netherlands Univ Twente POB 217 Enschede Netherlands NL-7500 AE therlands
Citazione:
M.L. Bennink et al., "Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers", NAT ST BIOL, 8(7), 2001, pp. 606-610

Abstract

Single chromatin fibers were assembled directly in the flow cell of an optical tweezers setup. A single h phage DNA molecule, suspended between two polystyrene beads, was exposed to a Xenopus laevis egg extract, leading to chromatin assembly with concomitant apparent shortening of the DNA molecule. Assembly was force-dependent and could not take place at forces exceeding 10 pN, The assembled single chromatin fiber was subjected to stretching by controlled movement of one of the beads with the force generated in the molecule continuously monitored with the second bead trapped in the optical trap. The force displayed discrete, sudden drops upon fiber stretching, reflecting discrete opening events in fiber structure. These opening events werequantized at increments in fiber length of similar to 65 mn and are attributed to unwrapping of the DNA from around individual histone octamers, Repeated stretching and relaxing of the fiber in the absence of egg extract showed that the loss of histone octamers was irreversible. The forces measuredfor Individual nucleosome disruptions are in the range of 20-40 pN, comparable to forces reported for RNA- and DNA-polymerases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:25:40