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Titolo:
Gene transfer into baboon repopulating cells: A comparison of Flt-3 ligandand megakaryocyte growth and development factor versus IL-3 during ex vivotransduction
Autore:
Kurre, P; Morris, J; Horn, PA; Harkey, MA; Andrews, RG; Kiem, HP;
Indirizzi:
Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Univ Washington, Sch Med, Dept Pediat, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Dept Pediat, Seattle, WA 98195 USA Univ Washington, Sch Med, Dept Med, Seattle, WA 98195 USA Univ WashingtonSeattle WA USA 98195 Med, Dept Med, Seattle, WA 98195 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 6, volume: 3, anno: 2001,
pagine: 920 - 927
SICI:
1525-0016(200106)3:6<920:GTIBRC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; COLONY-STIMULATING FACTOR; FIBRONECTIN FRAGMENT CH-296; MARROW PROGENITOR CELLS; RHESUS PERIPHERAL-BLOOD; RETROVIRAL TRANSDUCTION; NEGATIVE REGULATION; STROMAL SUPPORT; CD34(+) CELLS; KIT-LIGAND;
Keywords:
gene transfer; MGDF; Flt3-L; repopulating cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kiem, HP Fred Hutchinson Canc Res Ctr, Div Clin Res, 1100 Fairview Ave N, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr 1100 Fairview Ave N Seattle WA USA 98109
Citazione:
P. Kurre et al., "Gene transfer into baboon repopulating cells: A comparison of Flt-3 ligandand megakaryocyte growth and development factor versus IL-3 during ex vivotransduction", MOL THER, 3(6), 2001, pp. 920-927

Abstract

Oncoretroviral vectors require division of target cells for successful transduction. In the case of hematopoietic repopulating cells this can be achieved by cytokine stimulation using growth factor combinations which facilitate gene transfer and maintain engraftment. Interleukin-3 (IL-3) has been widely used in growth factor combinations, although more recent data in the mouse showed reduced engraftment in the presence of IL-3. Here, we used a competitive repopulation assay to study the influence of IL-3 and the early acting cytokines megakaryocyte growth and development factor (MCDF) and Flt3-ligand (Flt3-L) on gene transfer efficiency during ex vivo transduction of hematopoietic repopulating cells. In a direct comparison, baboon CD34-enriched cells were transduced on CH-296 fibronectin fragment in the presence of either IL-6, stem cell factor (SCF), Flt3-L, and MCDF or IL-3, IL-6, andSCF. Animals were followed for up to 55 weeks, and analysis of peripheral blood leukocytes by semiquantitative polymerase chain reaction showed that both cytokine combinations achieved marking of repopulating cells. A trend toward increased gene marking, especially early after transplant (P = 0.06), was seen with the combination of IL-6, SCF, Flt3-L, and MCDF. However, the highest gene marking was achieved when IL-3 was combined with early acting cytokines, suggesting that the difference observed in this study was probably due to the addition of MGDF and Flt3-L and not due to a negative effect of IL-3 on engraftment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 01:50:32