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Titolo:
Automated 5 ' nuclease assay for detection of virulence factors in porcineEscherichia coli
Autore:
Frydendahl, K; Imberechts, H; Lehmann, S;
Indirizzi:
Danish Vet Lab, DK-1790 Copenhagen V, Denmark Danish Vet Lab Copenhagen Denmark V t Lab, DK-1790 Copenhagen V, Denmark Vet & Agrochem Res Ctr, B-1180 Brussels, Belgium Vet & Agrochem Res Ctr Brussels Belgium B-1180 B-1180 Brussels, Belgium Bundesinst Gesundheitlichen Verbraucherschutz & V, D-6818 Dessau, Germany Bundesinst Gesundheitlichen Verbraucherschutz & V Dessau Germany D-6818
Titolo Testata:
MOLECULAR AND CELLULAR PROBES
fascicolo: 3, volume: 15, anno: 2001,
pagine: 151 - 160
SICI:
0890-8508(200106)15:3<151:A5'NAF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HEAT-STABLE ENTEROTOXIN; FIMBRIAL SUBUNIT GENE; NUCLEOTIDE-SEQUENCE; EDEMA DISEASE; POSTWEANING DIARRHEA; ADHESIVE FIMBRIAE; EFFACING LESIONS; F107 FIMBRIAE; WEANED PIGS;
Keywords:
E. coil; pigs; postweaning diarrhoea; oedema disease; virulence factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Frydendahl, K Danish Vet Lab, Bulowsvej 27, DK-1790 Copenhagen V, Denmark Danish Vet Lab Bulowsvej 27 Copenhagen Denmark V V, Denmark
Citazione:
K. Frydendahl et al., "Automated 5 ' nuclease assay for detection of virulence factors in porcineEscherichia coli", MOL CELL PR, 15(3), 2001, pp. 151-160

Abstract

This paper describes the development of a 5' nuclease assay for detection of virulence factor genes responsible for colonization factors and toxins in Escherichia coil isolated from pigs. Colonization factors were F4, F5, F6, F18, F41 and the outer membrane protein intimin. Toxins were heat stable (STa, STb, EAST1) and heat labile LT) enterotoxins and the verocytotoxin variant 2e (VT2e). To correctly identify false negative results, an endogenous internal control targeting the E. coil 16S rRNA gene was incorporated in each test tube. The assay was evaluated using a collection of E. coil reference strains which have previously been examined with phenotypical assays or DNA hybridization. Furthermore, the assay was evaluated by testing porcine E. coil field strains, previously characterized. The 5' nuclease assay correctly detected the presence of virulence genes in all reference strains. When testing field strains there was generally excellent agreement with results obtained by laboratories in Belgium and Germany. In conclusion, the 5'nuclease assay developed is a fast and specific tool for detection of E. coli virulence genes in the veterinary diagnostic laboratory. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 20:31:36