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Titolo:
Typing of IS1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp paratuberculosis distinct from that occurring in cattle and other domesticated livestock
Autore:
Whittington, RJ; Marsh, IB; Whitlock, RH;
Indirizzi:
NSW Agr, Elizabeth Macarthur Agr Inst, Camden, NSW 2570, Australia NSW Agr Camden NSW Australia 2570 r Agr Inst, Camden, NSW 2570, Australia Univ Penn, Sch Vet Med, New Bolton Ctr, Kennett Square, PA 19348 USA Univ Penn Kennett Square PA USA 19348 n Ctr, Kennett Square, PA 19348 USA
Titolo Testata:
MOLECULAR AND CELLULAR PROBES
fascicolo: 3, volume: 15, anno: 2001,
pagine: 139 - 145
SICI:
0890-8508(200106)15:3<139:TOIPCT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESTRICTION ENDONUCLEASE ANALYSIS; AMERICAN WILD RUMINANTS; PARA-TUBERCULOSIS; SHEEP; DEER; IDENTIFICATION; HYBRIDIZATION; ELK;
Keywords:
Mycobacterium avium subspecies paratuberculosis; IS900; IS1311; diagnosis; PCR; cattle; bison;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Whittington, RJ NSW Agr, Elizabeth Macarthur Agr Inst, PMB 8, Camden, NSW 2570, Australia NSW Agr PMB 8 Camden NSW Australia 2570 SW 2570, Australia
Citazione:
R.J. Whittington et al., "Typing of IS1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp paratuberculosis distinct from that occurring in cattle and other domesticated livestock", MOL CELL PR, 15(3), 2001, pp. 139-145

Abstract

Isolates of Mycobacterium avium subsp. paratuberculosis from nine bison (Bison bison) from Montana, United States of America, were compared with those from other species from the United States of America. All 16 isolates hada novel IS1311 genotype in which all copies of the element possessed a thymidine-cytosine nucleotide variation at base position 223. Isolates from bison were termed B strain. Thirteen isolates from cattle and goats were polymorphic at this locus, a status previously recognised in isolates from cattle which are termed C strains. Differences in cultural phenotype between B and C strains which were noted upon primary isolation did not manifest uponsubculture to Herrold's egg yolk medium or modified Middlebrook 7H10 agar. Rapid differentiation of these strains from each other and from S strains from sheep is possible using polymerase chain reaction and restriction endonuclease analysis. The epidemiology of paratuberculosis in bison in Montanaappears to be distinct from that in cattle and other farmed livestock investigated thus far. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 11:21:24