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Titolo:
Modulation of gene expression by androgen and oestrogens in the testis andprostate of the adult rat following androgen withdrawal
Autore:
Turner, KJ; Morley, M; MacPherson, S; Millar, MR; Wilson, JA; Sharpe, RM; Saunders, PTK;
Indirizzi:
MRC, Ctr Reprod Biol, Human Reprod Sci Unit, Edinburgh EH3 9ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9ET h EH3 9ET, Midlothian, Scotland
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 178, anno: 2001,
pagine: 73 - 87
SICI:
0303-7207(20010610)178:1-2<73:MOGEBA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN-RECEPTOR-BETA; VENTRAL PROSTATE; BINDING-PROTEIN; MESSENGER-RNA; REPRODUCTIVE-TRACT; HOMEOBOX GENE; SERTOLI CELLS; MICE LACKING; GERM-CELLS; ER-BETA;
Keywords:
testis; prostate; androgen receptor; oestrogen receptor beta; prostatein/C3; SGP-2/clusterin; EDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Saunders, PTK MRC, Ctr Reprod Biol, Human Reprod Sci Unit, 37 Chalmers St,Edinburgh EH39ET, Midlothian, Scotland MRC 37 Chalmers St Edinburgh Midlothian Scotland EH3 9ET and
Citazione:
K.J. Turner et al., "Modulation of gene expression by androgen and oestrogens in the testis andprostate of the adult rat following androgen withdrawal", MOL C ENDOC, 178(1-2), 2001, pp. 73-87

Abstract

Androgens are important for the structural and functional integrity of thetestis and the prostate and this may in part be mediated by the aromatisation of testosterone to oestradiol. The aim of the present study was to establish an in vivo model that would allow the identification of genes, the expression of which was regulated acutely by androgen and/or oestrogen in themale reproductive system. In rats in which the Leydig cells were ablated by administration of ethane dimethane sulfonate (EDS) 6 days earlier., testosterone esters (T) were administered from day 0 (To), and additional animals were administered either T, 17 beta -oestradiol benzoate (EB) or diethylstilbestrol (DES) for 1 or 4 h on day 6 after EDS-treatment. Nuclear immunoexpression of the androgen receptor. (AR) was reduced or absent from the testis but unaffected in the ventral prostate following these treatments. ER beta immunoexpression in these tissues was unchanged. Northern blot analysisshowed that EB and DES as well as T administration 4 h cal licl could modulate mRNA expression of two androgen-responsive genes, C3 and SGP-2, in theprostate. The co-administration of T or EB with the AR antagonist, flutamide, or with the ER antagonist, ICI 182,780 (ICI), did not block the suppression of SGP-2 mRNA expression by T or EB. In contrast, the upregulation of C3 mRNA expression by T was successfully antagonised by both flutamide and by ICI. A preliminary evaluation of the expression of three Sertoli cell and five germ cell mRNAs revealed that their expression was not steroid regulated. Our results support the hypothesis that the action of testosterone inthe male reproductive system may in part be mediated by its conversion to oestradiol. This in vivo model should prove of value in future studies to identify androgen and oestrogen regulated genes in the male reproductive system. (C) 2001 Elsevier Science ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:31:57