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Titolo:
Production of extracellular enzymes by Brevibacterium linens in fermenter and preparation of enzyme powder
Autore:
Ghosh, BC; Sienkiewicz, T; Pfaffe, E; Krenkel, K;
Indirizzi:
Berlin Univ Technol, Fac Proc Sci 3, Dept Fat Rich Prod, D-13355 Berlin, Germany Berlin Univ Technol Berlin Germany D-13355 Prod, D-13355 Berlin, Germany
Titolo Testata:
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL
fascicolo: 6, volume: 56, anno: 2001,
pagine: 332 - 335
SICI:
0026-3788(2001)56:6<332:POEEBB>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; PROTEINASE; CHEESE;
Keywords:
Brevibacterium linens (enzyme production, enzyme powder);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Ghosh, BC Natl Dairy Res Inst, Dairy Technol Div, Bangalore 560030, Karnataka, India Natl Dairy Res Inst Bangalore Karnataka India 560030 aka, India
Citazione:
B.C. Ghosh et al., "Production of extracellular enzymes by Brevibacterium linens in fermenter and preparation of enzyme powder", MILCHWISSEN, 56(6), 2001, pp. 332-335

Abstract

Two strains of Brevibacterium linens (B. linens FLK61 and B, linens DSM20425) were grown separately in a fermenter at 25 degreesC. Growth and extracellular proteinase production were analysed during fermentation. The rate ofgrowth was higher for DSM compared to FLK strain. Extracellular proteinaseactivity was slightly higher in culture of DSM after 19 h of fermentation. Cells were removed from the cultures by filtration at the end of 26 and 21h of fermentation for FLK and DSM strains, respectively. Cell free filtrates were concentrated 5fold by ultrafiltration (UF) and diafiltration. The retention of proteinase activity was 57.7 and 65.3% in UF and 48.0 and 51.4%in diafiltrated retentate for the enzymes from FLK and DSM strains, respectively. The yields of activity in lyophilised enzyme powders were 54.7 and 62.5% from UF and 47.1 and 51.2% from diafiltrated retentates of FLK and DSM strains, respectively. After 5 months of storage at -19 degreesC the lossin proteinase activity in the powder of ultrafiltrated enzymes from FLK and DSM strains amounted to 2.3 and 7.3%, respectively. For both enzymes the loss in activity during storage was lower in powder prepared from diafiltrated than ultrafiltrated retentate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:58:22